Comparison of Extraction Methods for the Detection of Avian Influenza Virus RNA in Cattle Milk
Abstract
:1. Introduction
2. Materials and Methods
2.1. Milk Samples
2.2. Amplification of Virus Stocks
2.3. Artificial Sample Contamination
2.4. Viral RNA Extraction
2.5. Reverse Transcription Quantitative Real-Time PCR (RT-qPCR)
2.6. Data Analysis
3. Results
3.1. Comparison of Extraction Methods Using Bulk Tank Milk
3.2. Comparison of Extraction Methods Using Individual Milk Samples
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Snoeck, C.J.; Sausy, A.; Bourg, M.; Hübschen, J.M. Comparison of Extraction Methods for the Detection of Avian Influenza Virus RNA in Cattle Milk. Viruses 2024, 16, 1442. https://doi.org/10.3390/v16091442
Snoeck CJ, Sausy A, Bourg M, Hübschen JM. Comparison of Extraction Methods for the Detection of Avian Influenza Virus RNA in Cattle Milk. Viruses. 2024; 16(9):1442. https://doi.org/10.3390/v16091442
Chicago/Turabian StyleSnoeck, Chantal J., Aurélie Sausy, Manon Bourg, and Judith M. Hübschen. 2024. "Comparison of Extraction Methods for the Detection of Avian Influenza Virus RNA in Cattle Milk" Viruses 16, no. 9: 1442. https://doi.org/10.3390/v16091442