Peer-Review Record
Progesterone Metabolism by Human and Rat Hepatic and Intestinal Tissue
Pharmaceutics 2021, 13(10), 1707; https://doi.org/10.3390/pharmaceutics13101707
by
Zoe Coombes 1
, Katie Plant 2, Cristina Freire 3, Abdul W. Basit 4, Philip Butler 2, R. Steven Conlan 1 and Deyarina Gonzalez 1,*
, Katie Plant 2, Cristina Freire 3, Abdul W. Basit 4, Philip Butler 2, R. Steven Conlan 1 and Deyarina Gonzalez 1,*Reviewer 1:
Anonymous
Reviewer 2:
Anonymous
Pharmaceutics 2021, 13(10), 1707; https://doi.org/10.3390/pharmaceutics13101707
Submission received: 10 September 2021
/
Revised: 5 October 2021
/
Accepted: 12 October 2021
/
Published: 16 October 2021
(This article belongs to the Special Issue Pre-clinical Pharmacology and Pharmacokinetics in Oral Drug Delivery)
Round 1
Reviewer 1 Report
Coombes et al provides an insightful study on progesterone metabolism in both intestinal and hepatic tissues. The paper is well written and interesting to read. There are a few comments that I think may improve the paper.
- Could you provide the cat numbers of all the materials used, including the inhibitors.
- Were any desalting or SPE clean up methods used for these samples prior to LC-MS? Is so could a description be included.
- A description outlining why the dosages of NADPH (1 mM) and inhibitors (1oo uM) were chosen, is 100 uM physiologically relevant for all the inhibitors used.
- Minor comment: the spacing between numbers and units is inconsistent eg lines 130 and 132
- Could a sample spectra be included in the appendix
- The figures should be presented in Graphpad is the analysis was performed with this software.
Author Response
Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
I congratulate the Authors on an interesting publication. I propose some corrections and additions to increase the value of the manuscript.
- What software used to calculate statistics. What test was used to check the distribution of variables?
- Minor errors, eg no space between the number and the value 8μl (make it uniform), incorrect entries: at 4oC, 37oC; (t1 / 2) ½ should be subscript.
- What was the short-term and long-term stability in room temperature and in the freezer for metoprolol?
- Parts are missing: discussion, after the results there is a summary.
- What are the limitations of the study?
- How can we use the obtained results in clinical practice?
Author Response
Please see attachment.
Author Response File: Author Response.docx
