Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Methods
2.2.1. Preparation of the Polymeric Blends
2.2.2. Preparation of Electrospun Scaffolds
2.2.3. Scaffold Chemical–Physical Characterization
2.2.4. Scaffold Mechanical Properties
2.2.5. Local Dynamics in Scaffolds
2.2.6. Platelet Lysate Release
2.2.7. Cytocompatibility, Adhesion, and Proliferation Assay: TEN-1 and SAOS-2 Cell Cultures
2.2.8. Alamar Blue Assay
2.2.9. Immunofluorescence Analysis
2.2.10. Statistical Analysis
3. Results and Discussion
3.1. Alignment and HP-Doping Affect the Scaffold Chemical–Physical Properties
3.2. Alignment and HP-Doping Modulate the Scaffold Mechanical Properties on the Macroscopic and Molecular Scales
3.3. HP-Doped Scaffolds Can Control the Release of Bioenhancer Proteins
3.4. HP-Doped Tubular Scaffolds Sustain Prolonged Bio-Enhanced Cell Proliferation
3.5. Anisotropy, HP-Doping, and PL-Bioenhancing Synergistically Aid Proper Morphology and ECM Production of Tendon and Bone Cells
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
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Scaffold | Pullulan % (w/w) | Chitosan % (w/w) | Citric Acid % (w/w) | HP % (w/w) |
---|---|---|---|---|
Blank | 66.8 | 16.6 | 16.6 | |
HP0.1 | 66.3 | 16.5 | 16.5 | 0.7 |
HP0.5 | 64.6 | 16.1 | 16.1 | 3.2 |
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Faccendini, A.; Bianchi, E.; Ruggeri, M.; Vigani, B.; Perotti, C.; Pavesi, F.C.; Caliogna, L.; Natali, F.; Del Favero, E.; Cantu’, L.; et al. Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface. Pharmaceutics 2021, 13, 1996. https://doi.org/10.3390/pharmaceutics13121996
Faccendini A, Bianchi E, Ruggeri M, Vigani B, Perotti C, Pavesi FC, Caliogna L, Natali F, Del Favero E, Cantu’ L, et al. Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface. Pharmaceutics. 2021; 13(12):1996. https://doi.org/10.3390/pharmaceutics13121996
Chicago/Turabian StyleFaccendini, Angela, Eleonora Bianchi, Marco Ruggeri, Barbara Vigani, Cesare Perotti, Francesco Claudio Pavesi, Laura Caliogna, Francesca Natali, Elena Del Favero, Laura Cantu’, and et al. 2021. "Smart Device for Biologically Enhanced Functional Regeneration of Osteo–Tendon Interface" Pharmaceutics 13, no. 12: 1996. https://doi.org/10.3390/pharmaceutics13121996