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Pharmaceutics
Volume 13
Issue 12
10.3390/pharmaceutics13122153
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Article

Versatile and Robust Method for Antibody Conjugation to Nanoparticles with High Targeting Efficiency

by
Indra Van Zundert
1,
Maria Bravo
1,
Olivier Deschaume
2,
Pierre Cybulski
1,
Carmen Bartic
2,
Johan Hofkens
1,
Hiroshi Uji-i
1,3,
Beatrice Fortuni
1,* and
Susana Rocha
1,*
1
Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Celestijnenlaan 200F, 3001 Heverlee, Belgium
2
Soft-Matter Physics and Biophysics, Department of Physics and Astronomy, KU Leuven, Celestijnenlaan 200D, Box 2416, 3001 Heverlee, Belgium
3
Research Institute for Electronic Science (RIES), Hokkaido University, N20W10, Kita Ward, Sapporo 001-0020, Japan
*
Authors to whom correspondence should be addressed.
Pharmaceutics 2021, 13(12), 2153; https://doi.org/10.3390/pharmaceutics13122153
Submission received: 28 September 2021 / Revised: 7 December 2021 / Accepted: 8 December 2021 / Published: 14 December 2021
(This article belongs to the Special Issue Novel Anticancer Strategies (Volume II))
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Abstract

The application of antibodies in nanomedicine is now standard practice in research since it represents an innovative approach to deliver chemotherapy agents selectively to tumors. The variety of targets or markers that are overexpressed in different types of cancers results in a high demand for antibody conjugated-nanoparticles, which are versatile and easily customizable. Considering up-scaling, the synthesis of antibody-conjugated nanoparticles should be simple and highly reproducible. Here, we developed a facile coating strategy to produce antibody-conjugated nanoparticles using ‘click chemistry’ and further evaluated their selectivity towards cancer cells expressing different markers. Our approach was consistently repeated for the conjugation of antibodies against CD44 and EGFR, which are prominent cancer cell markers. The functionalized particles presented excellent cell specificity towards CD44 and EGFR overexpressing cells, respectively. Our results indicated that the developed coating method is reproducible, versatile, and non-toxic, and can be used for particle functionalization with different antibodies. This grafting strategy can be applied to a wide range of nanoparticles and will contribute to the development of future targeted drug delivery systems.
Keywords: mesoporous silica nanoparticles; antibody functionalization; targeted drug delivery systems mesoporous silica nanoparticles; antibody functionalization; targeted drug delivery systems
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MDPI and ACS Style

Van Zundert, I.; Bravo, M.; Deschaume, O.; Cybulski, P.; Bartic, C.; Hofkens, J.; Uji-i, H.; Fortuni, B.; Rocha, S. Versatile and Robust Method for Antibody Conjugation to Nanoparticles with High Targeting Efficiency. Pharmaceutics 2021, 13, 2153. https://doi.org/10.3390/pharmaceutics13122153

AMA Style

Van Zundert I, Bravo M, Deschaume O, Cybulski P, Bartic C, Hofkens J, Uji-i H, Fortuni B, Rocha S. Versatile and Robust Method for Antibody Conjugation to Nanoparticles with High Targeting Efficiency. Pharmaceutics. 2021; 13(12):2153. https://doi.org/10.3390/pharmaceutics13122153

Chicago/Turabian Style

Van Zundert, Indra, Maria Bravo, Olivier Deschaume, Pierre Cybulski, Carmen Bartic, Johan Hofkens, Hiroshi Uji-i, Beatrice Fortuni, and Susana Rocha. 2021. "Versatile and Robust Method for Antibody Conjugation to Nanoparticles with High Targeting Efficiency" Pharmaceutics 13, no. 12: 2153. https://doi.org/10.3390/pharmaceutics13122153

APA Style

Van Zundert, I., Bravo, M., Deschaume, O., Cybulski, P., Bartic, C., Hofkens, J., Uji-i, H., Fortuni, B., & Rocha, S. (2021). Versatile and Robust Method for Antibody Conjugation to Nanoparticles with High Targeting Efficiency. Pharmaceutics, 13(12), 2153. https://doi.org/10.3390/pharmaceutics13122153

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