A Recombinant Fusion Construct between Human Serum Albumin and NTPDase CD39 Allows Anti-Inflammatory and Anti-Thrombotic Coating of Medical Devices
Abstract
:1. Introduction
2. Materials and Methods
2.1. Generation of Recombinant Fusion Construct, Production, Expression of Protein and Purification
2.2. Blood Sampling from Healthy Human Volunteers
2.3. Preparation of Platelet-Rich Plasma
2.4. Flow Cytometry
2.5. ADP Bioluminescence Assay
2.6. Lyophilisation of Protein
2.7. Coating of Stent Material for In Vitro Analysis
2.8. Endothelialisation Analysis of Protein-Coated Nitinol BlueOxide Plates
2.9. In Vitro Haemocompatibility Testing Using Roller Pump and Modified Chandler Loop Model
2.10. Statistical Analysis
3. Results
3.1. Generation, Production and Enzymatic Activity of Recombinant Fusion Protein HSA-CD39
3.2. In Vitro Analysis of Environmental Stability of HSA-CD39 Fusion Protein and Coating
3.3. HSA-CD39 Fusion Protein as an Anti-Thrombotic Coating for Different Medical Materials
3.4. HSA-CD39 Fusion Protein Coating Allows for Endothelialisation
3.5. Haemocompatibility and In Vitro Proof of Function of HSA-CD39-Coated Nitinol Blue Oxide Stents and PVC Tubes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Abraham, M.-K.; Jost, E.; Hohmann, J.D.; Searle, A.K.; Bongcaron, V.; Song, Y.; Wendel, H.P.; Peter, K.; Krajewski, S.; Wang, X. A Recombinant Fusion Construct between Human Serum Albumin and NTPDase CD39 Allows Anti-Inflammatory and Anti-Thrombotic Coating of Medical Devices. Pharmaceutics 2021, 13, 1504. https://doi.org/10.3390/pharmaceutics13091504
Abraham M-K, Jost E, Hohmann JD, Searle AK, Bongcaron V, Song Y, Wendel HP, Peter K, Krajewski S, Wang X. A Recombinant Fusion Construct between Human Serum Albumin and NTPDase CD39 Allows Anti-Inflammatory and Anti-Thrombotic Coating of Medical Devices. Pharmaceutics. 2021; 13(9):1504. https://doi.org/10.3390/pharmaceutics13091504
Chicago/Turabian StyleAbraham, Meike-Kristin, Elena Jost, Jan David Hohmann, Amy Kate Searle, Viktoria Bongcaron, Yuyang Song, Hans Peter Wendel, Karlheinz Peter, Stefanie Krajewski, and Xiaowei Wang. 2021. "A Recombinant Fusion Construct between Human Serum Albumin and NTPDase CD39 Allows Anti-Inflammatory and Anti-Thrombotic Coating of Medical Devices" Pharmaceutics 13, no. 9: 1504. https://doi.org/10.3390/pharmaceutics13091504