Initial Steps towards Spatiotemporal Signaling through Biomaterials Using Click-to-Release Chemistry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Type I Collagen Scaffolds
2.2. Reaction Components and Conditions
2.3. Ligation of hEGF to TCO and Release of hEGF following Tetrazine Exposure
2.4. Ligation of hEGF–TCO to Collagen Scaffolds and Release of hEGF following Tetrazine Exposure
2.4.1. SDS-PAGE and Western Blotting
2.4.2. Immunofluorescence Staining
2.5. Ligation of hEGF to BSA and Release following Tetrazine Exposure
3. Results
3.1. hEGF Ligates to TCO to Form an ‘hEGF–TCO’ Complex
3.2. The hEGF–TCO Complex Is Able to Ligate to Collagen Scaffolds
3.3. Establishing Click-to-Release of hEGF from BSA–TCO–hEGF
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gansevoort, M.; Merx, J.; Versteeg, E.M.M.; Vuckovic, I.; Boltje, T.J.; van Kuppevelt, T.H.; Daamen, W.F. Initial Steps towards Spatiotemporal Signaling through Biomaterials Using Click-to-Release Chemistry. Pharmaceutics 2022, 14, 1991. https://doi.org/10.3390/pharmaceutics14101991
Gansevoort M, Merx J, Versteeg EMM, Vuckovic I, Boltje TJ, van Kuppevelt TH, Daamen WF. Initial Steps towards Spatiotemporal Signaling through Biomaterials Using Click-to-Release Chemistry. Pharmaceutics. 2022; 14(10):1991. https://doi.org/10.3390/pharmaceutics14101991
Chicago/Turabian StyleGansevoort, Merel, Jona Merx, Elly M. M. Versteeg, Isidora Vuckovic, Thomas J. Boltje, Toin H. van Kuppevelt, and Willeke F. Daamen. 2022. "Initial Steps towards Spatiotemporal Signaling through Biomaterials Using Click-to-Release Chemistry" Pharmaceutics 14, no. 10: 1991. https://doi.org/10.3390/pharmaceutics14101991