Adjusting Heterodimeric Coiled-Coils (K/E Zipper) to Connect Autophagy-Inducing Peptide with Cell-Penetrating Peptide
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Antibodies
2.3. Peptide Synthesis
2.4. Fluorescence Titration Curves
2.5. Cell Culture
2.6. Preparation of the K/E Zippers
2.7. Evaluation of the K/E Hybrid Delivery into Cells
2.8. Analysis of Cellular Autophagic Activity
2.9. Cytotoxicity Assay
3. Results and Discussion
3.1. Chemical Structures of Fluorescently Labeled Hetrodimeric Coiled-Coil Modified with AIP or CPP
3.2. Comparison of Binding Constant of the AIP-Kn/En-CPP Hybrids
3.3. Intracellular Delivery of AIP-Kn/En-CPP Hybrids
3.4. Evaluation of Autophagy Induction by the Hybrid of AIP-Kn and En-CPP
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hakata, Y.; Yamashita, K.; Hashimoto, S.; Ohtsuki, T.; Miyazawa, M.; Kitamatsu, M. Adjusting Heterodimeric Coiled-Coils (K/E Zipper) to Connect Autophagy-Inducing Peptide with Cell-Penetrating Peptide. Pharmaceutics 2023, 15, 1048. https://doi.org/10.3390/pharmaceutics15041048
Hakata Y, Yamashita K, Hashimoto S, Ohtsuki T, Miyazawa M, Kitamatsu M. Adjusting Heterodimeric Coiled-Coils (K/E Zipper) to Connect Autophagy-Inducing Peptide with Cell-Penetrating Peptide. Pharmaceutics. 2023; 15(4):1048. https://doi.org/10.3390/pharmaceutics15041048
Chicago/Turabian StyleHakata, Yoshiyuki, Kazuma Yamashita, Sonoko Hashimoto, Takashi Ohtsuki, Masaaki Miyazawa, and Mizuki Kitamatsu. 2023. "Adjusting Heterodimeric Coiled-Coils (K/E Zipper) to Connect Autophagy-Inducing Peptide with Cell-Penetrating Peptide" Pharmaceutics 15, no. 4: 1048. https://doi.org/10.3390/pharmaceutics15041048