Tubeimoside-2 Triggers Methuosis in Hepatocarcinoma Cells through the MKK4–p38α Axis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
2.2. Cell Culture
2.3. Cell Viability Assay
2.4. Dextran/Lucifer Yellow Uptake Assay
2.5. Transmission Electron Microscopy
2.6. Proteomic Analysis
2.7. Western Blot
2.8. Total Cholesterol Assay
2.9. Xenograft Experiments
2.10. Statistical Analysis
3. Results
3.1. TBM-2 Treatment Induced Methuosis in Hepatocarcinoma Cells
3.2. TBM-2 Enhanced Lipid Metabolism to Support Methuosis
3.3. TBM-2 Treatment Hyperactivated the MAPK Signal Pathway
3.4. TBM-2 Induced Methuosis via the MKK4–p38α Axis
3.5. TBM-2 Triggered Methuosis and Suppressed Hepatocarcinoma Tumor Growth In Vivo
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gan, Y.; Wang, C.; Chen, Y.; Hua, L.; Fang, H.; Li, S.; Chai, S.; Xu, Y.; Zhang, J.; Gu, Y. Tubeimoside-2 Triggers Methuosis in Hepatocarcinoma Cells through the MKK4–p38α Axis. Pharmaceutics 2023, 15, 1093. https://doi.org/10.3390/pharmaceutics15041093
Gan Y, Wang C, Chen Y, Hua L, Fang H, Li S, Chai S, Xu Y, Zhang J, Gu Y. Tubeimoside-2 Triggers Methuosis in Hepatocarcinoma Cells through the MKK4–p38α Axis. Pharmaceutics. 2023; 15(4):1093. https://doi.org/10.3390/pharmaceutics15041093
Chicago/Turabian StyleGan, Yichao, Chen Wang, Yunyun Chen, Linxin Hua, Hui Fang, Shu Li, Shoujie Chai, Yang Xu, Jiawei Zhang, and Ying Gu. 2023. "Tubeimoside-2 Triggers Methuosis in Hepatocarcinoma Cells through the MKK4–p38α Axis" Pharmaceutics 15, no. 4: 1093. https://doi.org/10.3390/pharmaceutics15041093