Virus-like Silica Nanoparticles Improve Permeability of Macromolecules across the Blood–Brain Barrier In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Synthesis of Silica Nanoparticles
2.2.1. Virus-like Silica Nanoparticles (VSNP)
2.2.2. Stöber Silica Nanoparticles
2.3. Fluorescent Dye Cy5 Labelling on Silica Nanoparticles
2.4. Physiochemical Characterisation of Nanoparticles
2.5. Cell Culture
2.6. Cell Viability
2.7. Cellular Uptake
2.8. In Vitro Transwell BBB Model
2.9. Modulation of In Vitro BBB Model for Transport of Macromolecules
2.10. Statistical Analysis
3. Results and Discussion
3.1. Synthesis, Functionalization, and Characterization of Nanoparticles
3.2. Cell Viability and Cellular Uptake
3.3. Effect of Silica Nanoparticles on the Integrity of the Blood–Brain Barrier In Vitro
3.4. Transient Relaxation of Tight Junctions in the Blood–Brain Barrier for Macromolecule Delivery
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | Average Size nm (Measured via TEM) | BET Surface Area (m2g−1) |
---|---|---|
Stöber | 59 ± 6 | 78 |
VSNP | 62 ± 10 | 117 |
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Feng, Y.; Cao, Y.; Qu, Z.; Janjua, T.I.; Popat, A. Virus-like Silica Nanoparticles Improve Permeability of Macromolecules across the Blood–Brain Barrier In Vitro. Pharmaceutics 2023, 15, 2239. https://doi.org/10.3390/pharmaceutics15092239
Feng Y, Cao Y, Qu Z, Janjua TI, Popat A. Virus-like Silica Nanoparticles Improve Permeability of Macromolecules across the Blood–Brain Barrier In Vitro. Pharmaceutics. 2023; 15(9):2239. https://doi.org/10.3390/pharmaceutics15092239
Chicago/Turabian StyleFeng, Yuran, Yuxue Cao, Zhi Qu, Taskeen Iqbal Janjua, and Amirali Popat. 2023. "Virus-like Silica Nanoparticles Improve Permeability of Macromolecules across the Blood–Brain Barrier In Vitro" Pharmaceutics 15, no. 9: 2239. https://doi.org/10.3390/pharmaceutics15092239