Silver Nanoparticles In Situ Synthesized and Incorporated in Uniaxial and Core–Shell Electrospun Nanofibers to Inhibit Coronavirus
Abstract
:1. Introduction
2. Material and Methods
2.1. Materials
2.2. Methods
2.2.1. Preparation of Polymeric Solutions for Electrospinning
2.2.2. Uniaxial Electrospinning
2.2.3. Coaxial Electrospinning
2.2.4. Nanofiber Characterization
2.2.5. Mechanical Analysis
2.2.6. Disintegration Assay
2.2.7. Virucidal Assay
3. Results and Discussion
4. Virucidal Activity
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Solution | Material | %(w/V) | Solvent | Conditions |
---|---|---|---|---|
1 | PCL | 10.0 | DMF/DCM (1:1) | 1 h at 25 °C |
2 | CHT | 10.0 | H2O and 2% CH3COOH | 3 h at 60 °C |
3 | PVA | 12.0 | H2O | 3 h at 80 °C * |
4 | PVA-AgNO3 | 12.0; 0.25 | H2O | 3 h at 80 °C * |
Sample | Young’s Modulus (MPa) | Tensile Strength (10−3 MPa) | Elongation at Break (%) |
---|---|---|---|
PVA | 0.164 ± 0.008 | 10.8 ± 1.0 | 21.9 ± 6.9 |
PVA AgNPs | 0.084 ± 0.001 | 9.3 ± 0.10 | 113.6 ± 15.6 |
PVA/CHT | 0.020 ± 0.004 | 1.25 ± 0.10 | 6.2 ± 2.0 |
PVA/CHT/AgNPs | 0.030 ± 0.003 | 9.50 ± 0.05 | 15.5 ± 2.4 |
PCL[PVA/CHT] | 0.013 ± 0.005 | 1.45 ± 0.05 | 12.1 ± 0.3 |
PCL[PVA/CHT/AgNPs] | 0.049 ± 0.002 | 3.70 ± 0.70 | 16.1 ± 1.5 |
Time (h) | PVA | PVA/CHT | PCL[PVA/CHT] | AgNPs | PVA/AgNPs | PVA/CHT/AgNps | PCL[PVA/CHT/AgNPs] |
---|---|---|---|---|---|---|---|
Log ± SD | Log ± SD | Log ± SD | Log ± SD | Log ± SD | Log ± SD | Log ± SD | |
0.5 | 0.8 ± 0.5 | 1.5 ± 0.5 | 1.50 ± 0.5 | 5.3 ± 0.5 | 5.5 ± 0.5 | 5.3 ± 0.4 | 5.0 ± 0.7 |
1 | 1.0 ± 0.0 | 1.8 ± 0.4 | 1.50 ± 0.5 | 5.3 ± 0.5 | 5.5 ± 0.5 | 5.3 ± 0.4 | 5.3 ± 0.4 |
8 | 1.0 ± 0.4 | 2.0 ± 0.7 | 1.75 ± 0.4 | 5.8 ± 0.5 | 5.3 ± 0.4 | 5.5 ± 0.5 | 5.5 ± 0.5 |
24 | 0.8 ± 0.4 | 2.0 ± 0.0 | 2.00 ± 0.0 | 5.3 ± 0.5 | 5.5 ± 0.5 | 5.5 ± 0.5 | 5.5 ± 0.5 |
48 | 1.0 ± 0.0 | 2.0 ± 0.0 | 2.00 ± 0.0 | 6.5 ± 0.6 | 5.5 ± 0.5 | 5.5 ± 0.5 | 5.5 ± 0.5 |
64 | 1.0 ± 0.0 | 2.0 ± 0.0 | 2.00 ± 0.0 | 6.8 ± 0.5 | 5.5 ± 0.5 | 5.5 ± 0.5 | 5.5 ± 0.5 |
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de Freitas, C.F.; Souza, P.R.; Jacinto, G.S.; Braga, T.L.; Ricken, Y.S.; Souza, G.K.; Caetano, W.; Radovanovic, E.; Arns, C.W.; Rai, M.; et al. Silver Nanoparticles In Situ Synthesized and Incorporated in Uniaxial and Core–Shell Electrospun Nanofibers to Inhibit Coronavirus. Pharmaceutics 2024, 16, 268. https://doi.org/10.3390/pharmaceutics16020268
de Freitas CF, Souza PR, Jacinto GS, Braga TL, Ricken YS, Souza GK, Caetano W, Radovanovic E, Arns CW, Rai M, et al. Silver Nanoparticles In Situ Synthesized and Incorporated in Uniaxial and Core–Shell Electrospun Nanofibers to Inhibit Coronavirus. Pharmaceutics. 2024; 16(2):268. https://doi.org/10.3390/pharmaceutics16020268
Chicago/Turabian Stylede Freitas, Camila F., Paulo R. Souza, Gislaine S. Jacinto, Thais L. Braga, Yara S. Ricken, Gredson K. Souza, Wilker Caetano, Eduardo Radovanovic, Clarice W. Arns, Mahendra Rai, and et al. 2024. "Silver Nanoparticles In Situ Synthesized and Incorporated in Uniaxial and Core–Shell Electrospun Nanofibers to Inhibit Coronavirus" Pharmaceutics 16, no. 2: 268. https://doi.org/10.3390/pharmaceutics16020268