Next Article in Journal
Point of Care Testing of HIV in Children Younger Than 18 Months with Three Different HIV Virological Assays. Experience from a District Hospital in a Resource-Limited Setting
Previous Article in Journal
In Vitro Antimicrobial Activity of Salvadora persica Extract on Helicobacter pylori Strains Isolated from Duodenal Ulcer Biopsies
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Microbiology Research
Volume 3
Issue 1
10.4081/mr.2012.e13
microbiolres-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
Microbiology Research is published by MDPI from Volume 11 Issue 2 (2020). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Use of Immunodot Blot and Multiplex Reverse Transcriptase-Polymerase Chain Reaction in Dengue Virus Detection in Macerates of Aedes aegypti Larvae

by
Aline T.A. Chagas
1,
Michelle D. Oliveira
1,
Jose M.S. Mezencio
1,
Eduardo A.M. Silva
1,
Leandro L. Oliveira
1,
Eduardo Honda
2,
Cynthia C. da Silva
1 and
Sergio O. De Paula
1,*
1
Laboratory of Molecular Immunovirology, Federal University of Viçosa, Brazil
2
Center of Medicine in Tropical Medicine (CEPEM), Rondônia, Brazil
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2012, 3(1), e13; https://doi.org/10.4081/mr.2012.e13
Submission received: 13 October 2011 / Revised: 19 February 2012 / Accepted: 17 March 2012 / Published: 26 April 2012

Abstract

The Dengue virus is the main arbovirus that affects man in terms of morbidity and mortality. The detection of the virus is very important for epidemiological surveillance, so here we propose to standardize and compare the immunodot blot (IDB) and multiplex reverse transcriptase-polymerase chain reaction (M-RT-PCR) techniques to detect and characterize the dengue virus (DENV) serotypes in samples of Aedes aegypti larvae. Thus, the IDB and M-RT-PCR techniques were standardized using macerated samples of larvae collected in nature. The use of monoclonal antibodies in IDB has not shown great results, but DENV detection through this method was possible using polyclonal antibodies. The distinction of serotypes 1, 2 and 3 was carried out by M-RT-PCR.
Keywords: immunodot blot; multiplex reverse transcriptase-polymerase chain reaction; dengue control immunodot blot; multiplex reverse transcriptase-polymerase chain reaction; dengue control

Share and Cite

MDPI and ACS Style

Chagas, A.T.A.; Oliveira, M.D.; Mezencio, J.M.S.; Silva, E.A.M.; Oliveira, L.L.; Honda, E.; da Silva, C.C.; De Paula, S.O. Use of Immunodot Blot and Multiplex Reverse Transcriptase-Polymerase Chain Reaction in Dengue Virus Detection in Macerates of Aedes aegypti Larvae. Microbiol. Res. 2012, 3, e13. https://doi.org/10.4081/mr.2012.e13

AMA Style

Chagas ATA, Oliveira MD, Mezencio JMS, Silva EAM, Oliveira LL, Honda E, da Silva CC, De Paula SO. Use of Immunodot Blot and Multiplex Reverse Transcriptase-Polymerase Chain Reaction in Dengue Virus Detection in Macerates of Aedes aegypti Larvae. Microbiology Research. 2012; 3(1):e13. https://doi.org/10.4081/mr.2012.e13

Chicago/Turabian Style

Chagas, Aline T.A., Michelle D. Oliveira, Jose M.S. Mezencio, Eduardo A.M. Silva, Leandro L. Oliveira, Eduardo Honda, Cynthia C. da Silva, and Sergio O. De Paula. 2012. "Use of Immunodot Blot and Multiplex Reverse Transcriptase-Polymerase Chain Reaction in Dengue Virus Detection in Macerates of Aedes aegypti Larvae" Microbiology Research 3, no. 1: e13. https://doi.org/10.4081/mr.2012.e13

Article Metrics

Back to TopTop