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Microbiology Research
Volume 3
Issue 1
10.4081/mr.2012.e14
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Brief Report

Point of Care Testing of HIV in Children Younger Than 18 Months with Three Different HIV Virological Assays. Experience from a District Hospital in a Resource-Limited Setting

by
Gerardo Alvarez-Uria
1,*,
Jose M. Azcona
2,
Srinivasulu Reddy
2,
Manoranjan Midde
1,
Praveen K. Naik
1 and
N. Raghuprakash Reddy
2
1
Department of Infectious Diseases, India
2
Department of Microbiology. Rural Development Trust Hospital, Bathalapalli, Anantapur district, AP, India
*
Author to whom correspondence should be addressed.
Microbiol. Res. 2012, 3(1), e14; https://doi.org/10.4081/mr.2012.e14
Submission received: 26 October 2011 / Revised: 17 February 2012 / Accepted: 17 March 2012 / Published: 30 April 2012

Abstract

Diagnosis of HIV in children younger than 18 months can be challenging in developing countries because requires the use of HIV virological tests. In this study we describe the experience with three commercial assays, HIV-1 DNA polymerase chain reaction (PCR) (Roche Amplicor 1.5) with dried blood spot, HIV-1 RNA PCR (Roche COBAS TaqMan) with plasma and reverse transcriptase activity assay (Cavidi Exavir Load 3) with plasma in a rural setting of India. Sensitivity and specificity were 98.1% (95% confidence interval [CI] 90-100) and 99.3% (95% CI 97.9-99.9) for the HIV-1 RNA PCR assay, 66.7% (95% CI 29.9-92.5) and 100% (95% CI 96.8-100) for the HIV-1 DNA PCR assay, and 100% (95% CI 48-100) and 98.7% (95% CI 92.8-100) for the reverse transcriptase activity assay respectively. The low sensitivity of the HIV-1 DNA PCR assay in this setting is worrisome and warrants further investigations.
Keywords: HIV; pediatrics; diagnosis; polymerase chain reaction HIV; pediatrics; diagnosis; polymerase chain reaction

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MDPI and ACS Style

Alvarez-Uria, G.; Azcona, J.M.; Reddy, S.; Midde, M.; Naik, P.K.; Reddy, N.R. Point of Care Testing of HIV in Children Younger Than 18 Months with Three Different HIV Virological Assays. Experience from a District Hospital in a Resource-Limited Setting. Microbiol. Res. 2012, 3, e14. https://doi.org/10.4081/mr.2012.e14

AMA Style

Alvarez-Uria G, Azcona JM, Reddy S, Midde M, Naik PK, Reddy NR. Point of Care Testing of HIV in Children Younger Than 18 Months with Three Different HIV Virological Assays. Experience from a District Hospital in a Resource-Limited Setting. Microbiology Research. 2012; 3(1):e14. https://doi.org/10.4081/mr.2012.e14

Chicago/Turabian Style

Alvarez-Uria, Gerardo, Jose M. Azcona, Srinivasulu Reddy, Manoranjan Midde, Praveen K. Naik, and N. Raghuprakash Reddy. 2012. "Point of Care Testing of HIV in Children Younger Than 18 Months with Three Different HIV Virological Assays. Experience from a District Hospital in a Resource-Limited Setting" Microbiology Research 3, no. 1: e14. https://doi.org/10.4081/mr.2012.e14

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