International Journal of Plant Biology

21 pages, 655 KB

Open AccessReview

Unlocking the Potential of Biostimulants: A Review of Classification, Mode of Action, Formulations, Efficacy, Mechanisms, and Recommendations for Sustainable Intensification

by Unius Arinaitwe, Dalitso Noble. Yabwalo and Abraham Hangamaisho

Int. J. Plant Biol. 2025, 16(4), 122; https://doi.org/10.3390/ijpb16040122 (registering DOI) - 26 Oct 2025

Abstract

The escalating challenges of climate change, soil degradation, and the need to ensure global food security are driving the transition towards more sustainable agricultural practices. Biostimulants, a diverse category of substances and microorganisms, have emerged as promising tools to enhance crop resilience, improve [...] Read more.

The escalating challenges of climate change, soil degradation, and the need to ensure global food security are driving the transition towards more sustainable agricultural practices. Biostimulants, a diverse category of substances and microorganisms, have emerged as promising tools to enhance crop resilience, improve nutrient use efficiency (NUE), and support sustainable intensification. However, their widespread adoption is hampered by significant variability in efficacy and a lack of consensus on their optimal use. This comprehensive review synthesizes current scientific knowledge to critically evaluate the performance of biostimulants within sustainable agricultural systems. It aims to move beyond isolated case studies to provide a holistic analysis of their modes of action, efficacy under stress, and interactions with the environment. The analysis confirms that biostimulant efficacy is inherently context-dependent, governed by a complex interplay of biological, environmental, and management factors. Performance variability is explained by four core principles: the Limiting Factor Principle, the Biological Competition Axiom, the Stress Gradient Hypothesis, and the Formulation and Viability Imperative. A significant disconnect exists between promising controlled-environment studies and variable field results, highlighting the danger of extrapolating data without accounting for real-world agroecosystem complexity. Biostimulants are not universal solutions but are sophisticated tools whose value is realized through context-specific application. Their successful integration requires a precision-based approach aligned with specific agronomic challenges. We recommend that growers adopt diagnostic tools and on-farm trials, while producers must provide transparent multi-location field data and invest in advanced formulations. Future research must prioritize field validation, mechanistic studies using omics tools, and the development of crop-specific protocols and industry-wide standards to fully unlock the potential of biostimulants for building resilient and productive agricultural systems. Full article

(This article belongs to the Section Plant Response to Stresses)

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14 pages, 2195 KB

Open AccessArticle

Heritability of Morpho-Agronomic Traits in Cocona (Solanum sessiliflorum Dunal) and Efficiency of Early Visual Selection for Fruit Yield

by Leandro Sousa e Silva and César Augusto Ticona-Benavente

Int. J. Plant Biol. 2025, 16(4), 121; https://doi.org/10.3390/ijpb16040121 - 22 Oct 2025

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Abstract

Cocona (Solanum sessiliflorum Dunal) is an underutilized Amazonian fruit species with significant food, nutritional, and economic potential, but its genetic improvement remains limited. This study aimed to estimate the heritability of 13 morpho-agronomic traits in two F₂ populations, assess the efficiency [...] Read more.

Cocona (Solanum sessiliflorum Dunal) is an underutilized Amazonian fruit species with significant food, nutritional, and economic potential, but its genetic improvement remains limited. This study aimed to estimate the heritability of 13 morpho-agronomic traits in two F₂ populations, assess the efficiency of early visual selection, and identify traits associated with fruit yield. Approximately 250 plants from each population were grown in the Central Amazon. One week before the first harvest, plants were visually screened for yield potential, and the selected individuals were further evaluated for fruit traits. Broad-sense heritability (h²) was significant for most traits; the highest values were for number of flowers per plant (h² = 0.88), petiole length (h² = 0.87), collar diameter (h² = 0.71), canopy diameter (h² = 0.58), and fruit length (h² = 0.55). Early visual selection achieved ~65% efficiency. Fruit yield was correlated strongly and positively with the number of fruits per plant. These results indicate that phenotypic selection is effective for improving key plant and fruit traits in cocona. Early visual selection can be used to identify high-yielding individuals and the number of fruits per plant can be used as a complementary criterion to enhance selection accuracy for fruit yield. Full article

(This article belongs to the Section Plant Biochemistry and Genetics)

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15 pages, 1143 KB

Open AccessArticle

Adaptability and Phenotypic Stability of Early-Maturing Cowpea (Vigna unguiculata (L.) Walp.) Lines in the Peruvian Amazon

by Rodrigo Gonzales, César Augusto Ticona-Benavente, José Ramirez-Chung, Johnny Campos-Cedano and José Jesús Tejada-Alvarado

Int. J. Plant Biol. 2025, 16(4), 120; https://doi.org/10.3390/ijpb16040120 - 22 Oct 2025

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Abstract

This study evaluated the suitability of three floodplain environments near Iquitos for cowpea cultivar recommendations and estimated the adaptability and phenotypic stability of 12 cowpea lines evaluated in 2004. Climatic conditions between 2004 and 2020–2024 were also compared. Three field trials used a [...] Read more.

This study evaluated the suitability of three floodplain environments near Iquitos for cowpea cultivar recommendations and estimated the adaptability and phenotypic stability of 12 cowpea lines evaluated in 2004. Climatic conditions between 2004 and 2020–2024 were also compared. Three field trials used a randomized complete block design with 12 lines and two replications, assessing grain yield, number of pods per plant, days to flowering, days to 50% maturity, and days to harvest. Combined Analysis of Variance (ANOVA), Duncan’s means test, Genotype + Genotype Environment Interaction (GGE) biplot, and Additive Main Effects and Multiplicative Interactions Interaction (AMMI) analyses revealed that the three sites are contrasting. The Annicchiarico index, GGE biplot, and AMMI analyses identified line CAR 3010 as having superior adaptability and stability. Paired t-tests and Mann–Kendall analyses showed that climatic conditions in 2020–2024 differed significantly from 2004. Therefore, Muyuy, Rafael Belaunde, and San Miguel are suitable locations for testing advanced cowpea lines prior to cultivar recommendation. Line CAR 3010 is recommended for breeding programs in the Peruvian Amazon in response to ongoing climate change. This research addresses a critical knowledge gap as the first study evaluating cowpea adaptability and stability across multiple floodplain locations in the Peruvian Amazon. Full article

(This article belongs to the Section Plant Biochemistry and Genetics)

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19 pages, 989 KB

Open AccessArticle

Analysis of Phenotypic and Grain Quality Traits of Wheat Genotypes Under Drought-Stressed and Non-Stressed Conditions

by Maltase Mutanda and Sandiswa Figlan

Int. J. Plant Biol. 2025, 16(4), 119; https://doi.org/10.3390/ijpb16040119 - 15 Oct 2025

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Abstract

Wheat production and grain quality are adversely affected by drought stress. The deployment of wheat genotypes with improved grain yield and grain quality assists in achieving food security and maintaining a balanced diet. Therefore, this study is aimed at evaluating the phenotypic traits [...] Read more.

Wheat production and grain quality are adversely affected by drought stress. The deployment of wheat genotypes with improved grain yield and grain quality assists in achieving food security and maintaining a balanced diet. Therefore, this study is aimed at evaluating the phenotypic traits and grain quality responses of wheat genotypes to drought-stressed conditions. Two field trials were conducted to evaluate ten wheat genotypes under drought-stressed (DS) and non-stressed (NS) conditions in 2022 and 2023. The grains of the genotypes were further evaluated for their quality. The recorded phenotypic traits include grain yield (GY), shoot biomass (SB), root biomass (RB), and harvest index (HI). The grain quality traits recorded were grain carbon content (C), nitrogen (N), and crude protein (CP). Significant (p < 0.05) genetic variation were observed for the recorded phenotypic and grain quality traits. The highest grain yield was recorded in LM48 (495.83 g m⁻²), and the least was observed in BW141 (131.48 g m⁻²) under DS conditions. The N ranged from 1.76% recorded in LM75 to 3.16% (BW141) under DS conditions. The wheat genotypes, LM48 and BW140, presented high harvest index percentages, which indicates that the genotypes were efficient in partitioning their biomass to GY production even under DS conditions. The overall mean values of C and CP were lower under DS than NS conditions. Furthermore, GY was positively associated with SB (r = 0.50 under DS; r = 0.49 under NS) and RB (r = 0.38 under DS conditions; r = 0.32 under NS conditions). Amongst all the evaluated quality traits, only CP was negatively correlated with GY (r = −0.02) under DS conditions. Based on high GY production performance under DS conditions, the wheat genotypes LM48 and BW140 are recommended for further evaluation across diverse environments and production under limited water conditions. Full article

(This article belongs to the Topic Tolerance to Drought and Salt Stress in Plants, 2nd volume)

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28 pages, 5966 KB

Open AccessArticle

Evaluating the Impact of Mineral Nutrient Concentration and Substrate Volume on the Development of Three Annual Coastal Plant Species

by Astra Jēkabsone, Una Andersone-Ozola, Lidia Banaszczyk and Gederts Ievinsh

Int. J. Plant Biol. 2025, 16(4), 118; https://doi.org/10.3390/ijpb16040118 - 11 Oct 2025

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Abstract

Soil mineral nutrient heterogeneity is a distinctive characteristic of coastal habitats, yet its impact on plant growth and development remains uncertain. The objective of the present study was to establish an experimental system for evaluating the influence of mineral nutrient availability on the [...] Read more.

Soil mineral nutrient heterogeneity is a distinctive characteristic of coastal habitats, yet its impact on plant growth and development remains uncertain. The objective of the present study was to establish an experimental system for evaluating the influence of mineral nutrient availability on the development of three distinct short-lived wild coastal plant species: Phleum arenarium, Plantago coronopus, and Ranunculus sceleratus. These plants were cultivated in containers of different volumes employing an inert substrate with varying proportions of commercial garden soil in controlled conditions. Low mineral nutrient concentration served as a factor inhibiting plant vegetative growth for both P. arenarium and R. sceleratus plants, albeit with a substrate volume-dependent effect. In contrast, P. coronopus exhibited relatively low root biomass and exhibited minimal susceptibility to alterations in mineral nutrient concentration. Conversely, proportional allocation to roots decreased with increasing mineral nutrient concentration, mirroring the pattern observed for P. arenarium. Notably, for R. sceleratus, this effect was pronounced only at a high substrate volume. Furthermore, allocation to roots decreased with increasing substrate volume, but this occurred only at a high mineral nutrient concentration. The substrate, similar to that in coastal habitats, incorporated quartz sand with varying proportions of mineral-rich organic matter, providing comparable plant-available mineral concentrations for analyzing the effects of nutrient concentration, substrate volume, and genetic variability on plant growth and development. For future experiments, a wider range of mineral concentrations and more individual concentrations should be used to assess mineral availability more realistically. Full article

(This article belongs to the Section Plant Physiology)

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Graphical abstract

1 pages, 144 KB

Open AccessCorrection

Correction: Kolomeitseva et al. The Structural–Rhythmological Organization of Coelogyne (Orchidaceae Juss.) Inflorescences. Int. J. Plant Biol. 2023, 14, 286–298

by Galina Kolomeitseva, Vladimir Koval and Andrey Ryabchenko

Int. J. Plant Biol. 2025, 16(4), 117; https://doi.org/10.3390/ijpb16040117 - 10 Oct 2025

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Abstract

Following publication, concerns were raised to the editorial office relating to a potential conflict of interest between the authors and the Academic Editor that supervised the peer review of this article [...] Full article

(This article belongs to the Section Plant Physiology)

17 pages, 1009 KB

Open AccessArticle

Physiological and Transcriptome Analysis of Drought-Tolerant Mutant ds-1 of Blue Fescue (Festuca glauca) Under Drought Stress

by Yong Zhang, Peng Han, Xuefeng Xiao, Wei Chen, Hang Liu, Hengfeng Zhang and Lu Xu

Int. J. Plant Biol. 2025, 16(4), 116; https://doi.org/10.3390/ijpb16040116 - 4 Oct 2025

Viewed by 264

Abstract

Blue fescue (Festuca glauca) is a widely used ornamental grass worldwide. Drought is an important limiting factor for the growth and development of blue fescue; therefore, cultivating new strains of blue fescue with a strong drought tolerance is of great significance [...] Read more.

Blue fescue (Festuca glauca) is a widely used ornamental grass worldwide. Drought is an important limiting factor for the growth and development of blue fescue; therefore, cultivating new strains of blue fescue with a strong drought tolerance is of great significance for its production practice. To investigate the drought tolerance mechanism of ds-1, this study subjected both ds-1 and “Festina” to a natural drought treatment and measured their physiological and biochemical indicators. A transcriptomic analysis was also conducted to explore the underlying molecular mechanisms. The results showed that, after the drought treatment, the relative water content (RWC), water use efficiency (WUE), and photosynthetic rate (Pn) of ds-1 leaves were significantly higher than those of “Festina”; in addition, the contents of H₂O₂ and O₂⁻, the relative electrical conductivity (REC), the malondialdehyde (MDA) content, the gas conductance (Gs), and the transpiration rate (Tr) were significantly lower than those of “Festina”. The peroxidase (POD) activity of ds-1 was significantly higher than that of “Festina”, while the superoxide dismutase (SOD) activity of ds-1 was significantly lower than that of “Festina”. The transcriptome data analysis showed that there were a total of 9475 differentially expressed genes (DEGs) between ds-1 and “Festina”. A Venn plot analysis showed 692 DEGs between ds-1—8d vs. “Festina”—8d and ds-1—16d vs. “Festina”—16d. A KEGG enrichment analysis showed that these 692 genes were mainly enriched in 86 pathways, including those related to the photosynthesis antenna protein, plant hormone signal transduction, MAPK signaling, starch and sucrose metabolism, and arginine and proline metabolism. Further screening identified genes that may be associated with drought stress, including PYL, PP2C, SnRK2, ABF, BRI1, JAZ, MYC2, Lhc, and MPK6. The qRT-PCR results indicated that the expression trends of the DEGs were consistent with the transcriptome sequencing results. Our research results can provide a basis for exploring candidate genes for drought tolerance in blue fescue. In addition, our research results provide valuable genetic resources for the development of drought-resistant ornamental grass varieties, which can help reduce water consumption in cities and decrease labor and capital investment. Full article

(This article belongs to the Topic Tolerance to Drought and Salt Stress in Plants, 2nd volume)

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16 pages, 2557 KB

Open AccessArticle

Chitosan and Brassinosteroids Mitigate Ion Imbalance and Enhancing Stolon Production in Strawberry

by Miriam Elizabeth Martínez-Pérez, Dámaris Leopoldina Ojeda-Barrios, Rafael Ángel Parra-Quezada, Juan Luis Jacobo-Cuéllar, Brenda I. Guerrero, Esteban Sánchez-Chávez and Teresita de Jesús Ruíz-Anchondo

Int. J. Plant Biol. 2025, 16(4), 115; https://doi.org/10.3390/ijpb16040115 - 29 Sep 2025

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Abstract

Excess sodium in soil disrupts ionic balance and limits water uptake, negatively affecting growth and stolon production in strawberry plants. This study assessed the effects of chitosan (CTS), brassinosteroids (BRs), and thidiazuron (TDZ) on stolon performance and physiological responses of strawberry cv. ‘Portola’ [...] Read more.

Excess sodium in soil disrupts ionic balance and limits water uptake, negatively affecting growth and stolon production in strawberry plants. This study assessed the effects of chitosan (CTS), brassinosteroids (BRs), and thidiazuron (TDZ) on stolon performance and physiological responses of strawberry cv. ‘Portola’ under saline conditions. A greenhouse experiment included seven treatments: CTS, BRs, CTS + BRs combinations, TDZ, and an untreated control. Foliar applications were used to evaluate impacts on nutrient uptake, photosynthetic pigments, oxidative stress, and stolon production. BRs alone [2.53 × 10⁻⁶ μM] significantly increased crown diameter (+43%), stolon number (+65%), stolon length (+4%), and daughter plant formation (+8%), while reducing leaf sodium by 60% and improving Mg²⁺/Na⁺ and K⁺/Na⁺ ratios. The CTS + BRs combination enhanced phenolic content and produced the heaviest first daughter plants (6.1 g). TDZ, however, resulted in weaker stolons, lower chlorophyll a content, and reduced K⁺/Na⁺ ratios, suggesting a need for dose optimization. Overall, BRs, alone or with CTS, improved salt tolerance and stolon propagation through enhanced ion regulation, photosynthesis, and antioxidant defenses. These findings advance understanding of how biostimulants modulate metal ion homeostasis, antioxidant signaling, and growth in salt-sensitive crops, offering strategies to mitigate salinity stress in strawberry cultivation. Full article

(This article belongs to the Section Plant Response to Stresses)

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42 pages, 12138 KB

Open AccessArticle

Genome-Wide Metatranscriptomics Crosswalk of Diseased Common Beans (Phaseolus vulgaris L.) Unravels Critical Metabolic Pathways Involved in Plant Defense Mechanisms

by Aggrey Keya Osogo, Shrabana Sarkar, Francis Muyekho, Hassan Were and Patrick Okoth

Int. J. Plant Biol. 2025, 16(4), 114; https://doi.org/10.3390/ijpb16040114 - 28 Sep 2025

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Abstract

The common bean (Phaseolus vulgaris L.) is a vital food crop worldwide, particularly in Latin America, Asia, and Sub-Saharan Africa, due to its high levels of protein, fiber, and essential nutrients. However, it is susceptible to viral infections, especially from the Bean [...] Read more.

The common bean (Phaseolus vulgaris L.) is a vital food crop worldwide, particularly in Latin America, Asia, and Sub-Saharan Africa, due to its high levels of protein, fiber, and essential nutrients. However, it is susceptible to viral infections, especially from the Bean common mosaic virus and Bean common mosaic necrosis virus. While previous research has primarily focused on specific resistance genes, a broader understanding of the plant’s overall immune response remains limited. To investigate this, a study was conducted involving 51 infected leaf samples. RNA was extracted, and deep metatranscriptomic sequencing was performed using the Illumina MiSeq platform. The results indicated that several genes related to stress response, nitrogen metabolism, and biosynthesis pathways were activated during infection. Key defense mechanisms included pathogen recognition, the production of antimicrobial peptides, and changes in metabolic activity. The Mitogen-Activated Protein Kinase (MAPK) signaling pathway and enzymes like glycosyl transferases, which aid in building protective structures, played a significant role. These findings suggest that the bean’s defense system is complex and involves not only direct attacks on pathogens but also metabolic shifts and microbial interactions. Understanding these processes provides valuable insights for breeding stronger, disease-resistant, and climate-resilient bean varieties. Full article

(This article belongs to the Section Plant Biochemistry and Genetics)

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20 pages, 3754 KB

Open AccessArticle

Preliminary Evidence of Foliar Spray Effectiveness Against the Invasive Cactus Cylindropuntia pallida (Rose), F.M. Knuth in South Africa

by Keletso Makaota, Thabiso Michael Mokotjomela, Caswell Munyai, Thembelihle Joyce Mbele and Nontembeko Dube

Int. J. Plant Biol. 2025, 16(4), 113; https://doi.org/10.3390/ijpb16040113 - 25 Sep 2025

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Abstract

Using the biometric differences (i.e., plant physical characteristics) between the C. pallida populations previously treated with herbicide and those of the untreated populations, we tested the prediction that herbicidal treatment suppresses C. pallida plant growth in South Africa, where invasive cacti display high [...] Read more.

Using the biometric differences (i.e., plant physical characteristics) between the C. pallida populations previously treated with herbicide and those of the untreated populations, we tested the prediction that herbicidal treatment suppresses C. pallida plant growth in South Africa, where invasive cacti display high resilience to herbicide treatment. We also determined whether the surrounding communities knew of C. pallida invasion and whether they experienced any negative impacts. Overall, biometric analyses supported the study’s prediction because C. pallida plant height in treated populations was significantly lower than in untreated populations and before treatment. The average plant height of populations not treated with herbicide (i.e., experimental control) and those measured before treatment were not significantly different, but both were significantly greater than the heights of populations previously treated with herbicide. Similarly, the mean number of fruits, cladodes and juveniles per C. pallida plant treated with herbicide was significantly lower than in the untreated populations. We found a significant positive correlation between all measured parameters and C. pallida plant height. Out of 39 participants, 16 (41.0%) confirmed C. pallida presence in and around their properties, with 21 (53.9%) participants having experienced negative impacts directly and indirectly through fatal injuries on pets and livestock in the sampled sites. We confirmed the effectiveness of herbicide in suppressing the invasion of C. pallida and that people experience substantial negative impacts in areas where the species has established. Full article

(This article belongs to the Topic Plant Invasion)

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26 pages, 1279 KB

Open AccessReview

Drought Stress in Cassava (Manihot esculenta): Management Strategies and Breeding Technologies

by Maltase Mutanda, Assefa B. Amelework, Nzumbululo Ndou and Sandiswa Figlan

Int. J. Plant Biol. 2025, 16(4), 112; https://doi.org/10.3390/ijpb16040112 - 23 Sep 2025

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Abstract

Drought stress is a major constraint to cassava productivity, especially in drought-prone regions. Although cassava is considered drought-tolerant, prolonged or severe water scarcity significantly reduces tuber yield, carbon assimilation capacity and overall plant growth. The development, selection and deployment of cassava genotypes with [...] Read more.

Drought stress is a major constraint to cassava productivity, especially in drought-prone regions. Although cassava is considered drought-tolerant, prolonged or severe water scarcity significantly reduces tuber yield, carbon assimilation capacity and overall plant growth. The development, selection and deployment of cassava genotypes with enhanced drought tolerance and water use efficiency (WUE) will help to achieve food security. The ability of cassava genotypes to maintain productivity under drought stress is enhanced by drought-responsive genes that regulate stress-related proteins and metabolites, contributing to stomatal closure, osmotic adjustment, antioxidant defense, and efficient carbon assimilation. Therefore, this comprehensive review aimed to document: (i) the effects of drought stress on cassava’s physiological, biochemical and agronomic traits, and (ii) the mitigation strategies and breeding technologies that can improve cassava yield production, drought tolerance and WUE. The key traits discussed include stomatal regulation, chlorophyll degradation, source–sink imbalance, root system architecture and carbon allocation dynamics. In addition, the review presents advances in genomic, proteomic and metabolomic tools, and emphasizes the role of early bulking genotypes, drought tolerance indices, and multi-trait selection in developing cassava cultivars with enhanced drought tolerance, drought escape and drought avoidance mechanism. Therefore, the integration of these strategies will accelerate the development, selection and deployment of improved cassava varieties, which contribute to sustainable productivity and global food security under climate change. Full article

(This article belongs to the Topic Tolerance to Drought and Salt Stress in Plants, 2nd volume)

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Int. J. Plant Biol., Volume 16, Issue 4 (December 2025) – 11 articles

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