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Hematology Reports
Volume 10
Issue 1
10.4081/hr.2018.7435
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Hematology Reports is published by MDPI from Volume 14 Issue 1 (2022). Previous articles were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence, and they are hosted by MDPI on mdpi.com as a courtesy and upon agreement with PAGEPress.
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Article

Acute Monocytic Leukemia Diagnosed by Flow Cytometry Includes Acute Myeloid Leukemias with Weakly or Faintly Positive Non-Specific Esterase Staining

by
Yuriko Zushi
1,
Miho Sasaki
1,
Ayano Mori
1,
Toshiharu Saitoh
1,
Takae Goka
2,
Yumi Aoyama
3,
Yuta Goto
3,
Hiroko Tsunemine
3,
Taiichi Kodaka
3 and
Takayuki Takahashi
3,*
1
Departments of Cell Therapy, Shinko Hospital, 1-4-47, Wakihama-cho, Chuo-ku, Kobe 651-0072, Japan
2
Departments of Laboratory Medicine, Shinko Hospital, 1-4-47, Wakihama-cho, Chuo-ku, Kobe 651-0072, Japan
3
Departments of Hematology, Shinko Hospital, 1-4-47, Wakihama-cho, Chuo-ku, Kobe 651-0072, Japan
*
Author to whom correspondence should be addressed.
Hematol. Rep. 2018, 10(1), 7435; https://doi.org/10.4081/hr.2018.7435
Submission received: 5 October 2017 / Revised: 10 February 2018 / Accepted: 28 February 2018 / Published: 29 March 2018

Abstract

A diagnosis of acute monocytic leukemia (AML-M5) based on α-naphthyl butyrate esterase (α-NB) staining has some problems, because AML-M5 leukemic cells often show weak or faint positivity on α-NB staining. In these situations, some cases of AML-M5 tend to be misdiagnosed as AML-M0. Therefore, we evaluated the significance of weak or faint α-NB staining in AML-M5 diagnosed by flow cytometry (FCM). Nineteen AML cases in which leukemic cells were negative for naphthol AS-D chloroacetate esterase staining were studied. For FCM, we defined leukemic cells as having a monocytic nature when more than 10% of the leukemic cells were positive for at least one of the following antigens: CD4, CD11c, CD14, and CD64. The monocytic nature determined by FCM was consistent with positive or weak positivity on α-NB staining. Five of 6 cases in which leukemic cells exhibited faint positivity for α-NB staining could be diagnosed as AML-M5 by FCM, while negative α-NB staining was consistent with a diagnosis of AML-M0. These results suggest that AML-M5 should be taken into consideration even when leukemic cells are faintly positive for α-NB staining.
Keywords: AML-M5; AML-M0; flow cytom-etry; α-naphthyl butyrate esterase staining; surface monocytic antigen AML-M5; AML-M0; flow cytom-etry; α-naphthyl butyrate esterase staining; surface monocytic antigen

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MDPI and ACS Style

Zushi, Y.; Sasaki, M.; Mori, A.; Saitoh, T.; Goka, T.; Aoyama, Y.; Goto, Y.; Tsunemine, H.; Kodaka, T.; Takahashi, T. Acute Monocytic Leukemia Diagnosed by Flow Cytometry Includes Acute Myeloid Leukemias with Weakly or Faintly Positive Non-Specific Esterase Staining. Hematol. Rep. 2018, 10, 7435. https://doi.org/10.4081/hr.2018.7435

AMA Style

Zushi Y, Sasaki M, Mori A, Saitoh T, Goka T, Aoyama Y, Goto Y, Tsunemine H, Kodaka T, Takahashi T. Acute Monocytic Leukemia Diagnosed by Flow Cytometry Includes Acute Myeloid Leukemias with Weakly or Faintly Positive Non-Specific Esterase Staining. Hematology Reports. 2018; 10(1):7435. https://doi.org/10.4081/hr.2018.7435

Chicago/Turabian Style

Zushi, Yuriko, Miho Sasaki, Ayano Mori, Toshiharu Saitoh, Takae Goka, Yumi Aoyama, Yuta Goto, Hiroko Tsunemine, Taiichi Kodaka, and Takayuki Takahashi. 2018. "Acute Monocytic Leukemia Diagnosed by Flow Cytometry Includes Acute Myeloid Leukemias with Weakly or Faintly Positive Non-Specific Esterase Staining" Hematology Reports 10, no. 1: 7435. https://doi.org/10.4081/hr.2018.7435

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