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Sustainability
Volume 12
Issue 6
10.3390/su12062360
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Article

Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples

by
Philjae Kim
1,
Tae Joong Yoon
2 and
Sook Shin
2,3,*
1
Research & Development Division, National Science Museum, Daejeon 34143, Korea
2
Institute of Marine Life Resources, Sahmyook University, Seoul 01795, Korea
3
Department of Animal Biotechnology & Resource, Sahmyook University, Seoul 01795, Korea
*
Author to whom correspondence should be addressed.
Sustainability 2020, 12(6), 2360; https://doi.org/10.3390/su12062360
Submission received: 31 January 2020 / Revised: 16 March 2020 / Accepted: 16 March 2020 / Published: 18 March 2020
(This article belongs to the Special Issue Harmful Organisms and their Management for Sustainable Environment)
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Abstract

In marine environments, environmental DNA (eDNA) can be effectively detected and possibly quantified when combined with molecular techniques, as demonstrated by several recent studies. In this study, we developed a species-specific primer set and a probe to detect the distribution and biomass of an invasive hydrozoan in South Korea, Ectopleura crocea. These molecular markers were designed to amplify a 187 bp region based on mitochondrial cytochrome c oxidase subunit I (COI) of E. crocea and were tested on seawater samples from 35 Korean harbors in 2017. Of the 35 sites we investigated, only nine harbors returned positive detections when using traditional survey methods, while surveys based on the use of eDNA techniques detected E. crocea DNA in all seawater samples. These results suggest that eDNA surveys based on molecular techniques are more effective at identifying species distribution and estimating biomass than traditional surveys based on visual assessment of morphology.
Keywords: Ectopleura crocea; environmental DNA; invasive species; monitoring; species-specific markers; quantitative PCR Ectopleura crocea; environmental DNA; invasive species; monitoring; species-specific markers; quantitative PCR

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MDPI and ACS Style

Kim, P.; Yoon, T.J.; Shin, S. Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples. Sustainability 2020, 12, 2360. https://doi.org/10.3390/su12062360

AMA Style

Kim P, Yoon TJ, Shin S. Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples. Sustainability. 2020; 12(6):2360. https://doi.org/10.3390/su12062360

Chicago/Turabian Style

Kim, Philjae, Tae Joong Yoon, and Sook Shin. 2020. "Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples" Sustainability 12, no. 6: 2360. https://doi.org/10.3390/su12062360

APA Style

Kim, P., Yoon, T. J., & Shin, S. (2020). Environmental DNA and Specific Primers for Detecting the Invasive Species Ectopleura crocea (Hydrozoa: Anthoathecata) in Seawater Samples. Sustainability, 12(6), 2360. https://doi.org/10.3390/su12062360

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