Effect of Newly Synthesized Salts and Three Common Micropollutants on the Biochemical Activity of Nitrifiers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Statistical Elaboration of the Results
2.2. Chemicals
3. Results and Discussion
4. Conclusions
- We found that both SQAS and MPs have an inhibitory effect on the biochemical activity of nitrifiers and on the degree of inhibition. The influence of all the tested substances is much larger on NOB than on AOB, and smallest on the respiratory activity of heterotrophs.
- Among the examined SQAS, SQAS1, demonstrated the highest impact on the biochemical activity of the nitrifiers in the complete concentration range.
- Three tested MPs also presented an inhibitory effect on nitrification but influenced the activity of nitrifiers to a lower extent than SQAS. DCF inhibits both AOB and NOB; EE2 inhibits the process of nitrification more. In the case of 4-NP, nitrification is inhibited to a minimum extent.
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Boni, M.R.; Sbaffoni, S.; Tedesco, P.; Vaccari, M. Mass balance of emerging organic micropollutants in a small wastewater treatment plant. WIT Trans. Ecol. Environ. 2012, 164, 345–356. [Google Scholar] [CrossRef]
- Mastroianni, N.; López de Alda, M.; Barceló, D. Emerging organic contaminants in aquatic environments: State-of-the-art and recent scientific contributions. Contrib. Sci. 2010, 6, 193–197. [Google Scholar] [CrossRef]
- Boleda, R.M.; Galceran, T.M.; Ventura, F. Behavior of pharmaceuticals and drugs of abuse in a drinking water treatment plant (DWTP) using combined conventional and ultrafiltration and reverse osmosis (UF/RO) treatments. Environ. Pollut. 2011, 159, 1584–1591. [Google Scholar] [CrossRef]
- Rattier, M.; Reungoat, J.; Keller, J.; Gernjak, V. Removal of micropollutants during tertiary wastewater treatment by biofiltration: Role of nitrifiers and removal mechanisms. Water Res. 2014, 54, 89–99. [Google Scholar] [CrossRef]
- De Gusseme, B.; Pycke, B.; Hennebel, T.; Marcoen, A.; Vlaeminck, S.E.; Noppe, H.; Boon, N.; Verstraete, W. Biological removal of 17α-ethinylestradiol by a nitrifier enrichment culture in a membrane bioreactor. Water Res. 2009, 43, 2493–2500. [Google Scholar] [CrossRef]
- Norton, J.M.; Alzerreca, J.J.; Suwa, Y.; Klotz, M.G. Diversity of ammonia monooxygenase operon in autotrophic ammonia oxidizing bacteria. Arch. Microbiol. 2002, 177, 139–149. [Google Scholar] [CrossRef]
- Surmacz-Gorska, J.; Gernaey, K.; Demuynck, C.; Vanrolleghem, P.; Verstraete, W. Nitrification monitoring in activated sludge by oxygen uptake rate (OUR) measurements. Water Res. 1996, 30, 1228–1236. [Google Scholar] [CrossRef]
- Wang, J.; Gong, B.; Huang, W. Bacterial community structure in simultaneous nitrification, denitrification and organic matter removal process treating saline mustard tuber wastewater as revealed by 16S rRNA sequencing. Bioresour. Technol. 2017, 228, 31–38. [Google Scholar] [CrossRef] [PubMed]
- Zhang, X.; Zheng, S.; Xiao, X.; Wang, L.; Yin, Y. Simultaneous nitrification/denitrification and stable sludge/water separation achieved in a conventional activated sludge process with severe filamentous bulking. Bioresour. Technol. 2017, 226, 267–271. [Google Scholar] [CrossRef]
- McCarty, G. Modes of action of nitrification inhibitors. Biol. Fertil. Soils 1999, 29, 1–9. [Google Scholar] [CrossRef]
- Tatari, K.; Gülay, A.; Thamdrup, B.; Albrechtsen, H.J.; Smets, B.F. Challenges in using allylthiourea and chlorate as specific nitrification inhibitors. Chemosphere 2017, 182, 301–305. [Google Scholar] [CrossRef] [Green Version]
- Du, J.; Qi, W.; Niu, Q.; Hu, Y.; Zhang, Y.; Yang, M.; Li, Y.-Y. Inhibition and acclimation of nitrifiers exposed to erythromycin. Ecol. Eng. 2016, 94, 337–343. [Google Scholar] [CrossRef]
- Batt, A.L.; Kim, S.; Aga, D.S. Enhanced biodegradation of lopromide and trimethoprim in nitrifying activated sludge. Environ. Sci. Technol. 2006, 40, 7367–7373. [Google Scholar] [CrossRef] [PubMed]
- Maeng, S.K.; Choi, B.G.; Lee, K.T.; Song, K.G. Influences of solid retention time, nitrification and microbial activity on the attenuation of pharmaceuticals and estrogens in membrane bioreactors. Water Res. 2013, 47, 3151–3162. [Google Scholar] [CrossRef]
- Sathyamoorthy, S.; Chandran, K.; Ramsburg, C.A. Biodegradation and cometabolic modeling of selected betablockers during ammonia oxidation. Environ. Sci. Technol. 2013, 47, 12835–12843. [Google Scholar] [CrossRef]
- Tran, N.H.; Nguyen, V.T.; Urase, T.; Ngo, H.H. Role of nitrification in the biodegradation of selected artificial sweetening agents in biological wastewater treatment process. Bioresour. Technol. 2014, 161, 40–46. [Google Scholar] [CrossRef]
- Kim, J.Y.; Ryu, K.; Kim, E.J.; Choe, W.S.; Cha, G.C.; Yoo, I.-K. Degradation of bisphenol A and nonylphenol by nitrifying activated sludge. Process Biochem. 2007, 42, 1470–1474. [Google Scholar] [CrossRef]
- Roh, H.; Subramanya, N.; Zhao, F.; Yu, C.P.; Sandt, J.; Chu, K.H. Biodegradation potential of wastewater micropollutants by ammonia-oxidizing bacteria. Chemosphere 2009, 77, 1084–1089. [Google Scholar] [CrossRef]
- Blum, D.J.W.; Speece, R.E. A Database of Chemical Toxicity to Environmental Bacteria and Its Use in Interspecies Comparisons and Correlations. J. Water Pollut. Control Fed. 1991, 63, 198–207. [Google Scholar]
- Liwarska-Bizukojc, E.; Galamon, M.; Bernat, P. Kinetics of Biological Removal of the Selected Micropollutants and Their Effect on Activated Sludge Biomass. Water Air Soil Pollut. 2018, 229, 356. [Google Scholar] [CrossRef] [Green Version]
- Delbeke, E.I.P.; Roman, B.I.; Marin, G.B.; Van Geem, K.M.; Stevens, C.V. A new class of antimicrobial biosurfactants: Quaternary ammonium sophorolipids. Green Chem. 2015, 17, 3373–3377. [Google Scholar] [CrossRef]
- Gut, L.; Plaza, E.; Hultman, B. Oxygen Uptake Rate (OUR) Tests for Assessment of Nitrifying Activities in the Deammonification System, Integration and Optimization of Urban Sanitation System, KTH Land and Water Resources Engineering; Royal Institute of Technology: Stockholm, Sweden, 2006; pp. 119–128. [Google Scholar]
- APHA; AWWA; WEF. Standard Methods for the Examination of Water and Wastewater, 22nd ed.; American Public Health Association; American Works Water Association; Water Environment Federation: Washington, DC, USA, 2012. [Google Scholar]
- Park, J.S.; Lee, C.H. Removal of soluble COD by a biofilm formed on a membrane in a jet loop type membrane bioreactor. Water Res. 2005, 39, 4609–4622. [Google Scholar] [CrossRef]
- Zielińska, M.; Bernat, K.; Cydzik-Kwiatkowska, A.; Wojnowska-Baryła, I. Respirometric activity of activated sludge in sequencing batch reactor depending on substrate and dissolved oxygen concentration. Environ. Prot. Eng. 2012, 38, 41–49. [Google Scholar] [CrossRef]
- Liwarska-Bizukojć, E.; Olejnik, D.; Delbeke, E.I.P.; Van Geem, K.M.; Stevens, C.V. Evaluation of biological properties and fate in the environment of a new class of biosurfactants. Chemosphere 2018, 200, 561–568. [Google Scholar] [CrossRef] [PubMed]
- OECD Chemical Group. Activated Sludge, Respiration Inhibition Test, Method 209, OECD Revised Guidelines for Tests for Ready Biodegradability; OECD: Paris, France, 1984. [Google Scholar]
- Tomlinson, T.G.; Boon, A.G.; Trotman, C.A.N. Inhibition of nitrification in the activated sludge process of sewage disposal. J Appl. Bacteriol. 1996, 29, 266–291. [Google Scholar] [CrossRef] [PubMed]
- He, Y.J.; Bishop, P.L. Effect of acid orange 7 on nitrification process. J. Environ. Eng. 1994, 120, 108–121. [Google Scholar] [CrossRef]
- Philips, S.; Hendrikus, J.L.; Verstraete, W. Origin, causes and effects of increased nitrite concentrations in aquatic environments. Rev. Environ. Sci. Biotechnol. 2002, 1, 115–141. [Google Scholar] [CrossRef]
- Tchobanoglous, G.; Burton, F.L.; Stensel, H.D. Wastewater Engineering: Treatment and Reuse, 4th ed.; McGraw Hill: New York, NY, USA, 2003. [Google Scholar]
- Wilén, B.M. Variation in dissolved oxygen concentration and its effect on the activated sludge properties studied at a full scale wastewater treatment plant. In Proceedings of the IWA World Water Congress & Exhibition, Montreal, QC, Canada, 19–24 September 2010. [Google Scholar]
- Jayamohan, S.; Ohgaki, S.; Hanaki, K. Effect of DO on kinetics of nitrification. Water Supply 1988, 6, 141–150. [Google Scholar]
- Liwarska-Bizukojć, E.; Olejnik, D.; Gałamon, M.; Bernat, P. Adaptacja osadu czynnego do ścieków zawierających wybrane mikrozanieczyszczenia. Gaz Woda Tech. 2017, 91, 340–343. [Google Scholar] [CrossRef]
- Margot, J.; Lochmatter, S.; Barry, D.A.; Holliger, C. Role of ammonia-oxidizing bacteria in micropollutant removal from wastewater with aerobic granular sludge. Water Sci. Technol. 2016, 73, 564–575. [Google Scholar] [CrossRef]
- Falas, P.; Andersen, H.R.; Ledin, A.; la Cour Jansen, J. Impact of solid retention time and nitrification capacity on the ability of activated sludge to remove pharmaceuticals. Environ. Technol. 2012, 33, 865–872. [Google Scholar] [CrossRef] [PubMed]
Tested Compound | SOURTOT (mg O2 g VSS−1 h−1) | SOURAOB (mg O2 g VSS−1 h−1) | SOURNOB (mg O2 g VSS−1 h−1) | SOURHET (mg O2 g VSS−1 h−1) |
---|---|---|---|---|
SQAS1 | 4.86 | 0.54 | 0.50 | 3.82 |
SQAS2 | 5.76 | 1.26 | 0.50 | 4.00 |
SQAS3 | 4.86 | 0.36 | 0.40 | 4.10 |
SQAS4 | 4.68 | 0.18 | 0.15 | 4.35 |
None (Control) | 8.64 | 1.78 | 1.96 | 4.90 |
Tested Compound | SOURTOT (mg O2 g VSS−1 h−1) | SOURAOB (mg O2 g VSS−1 h−1) | SOURNOB (mg O2 g VSS−1 h−1) | SOURHET (mg O2 g VSS−1 h−1) |
---|---|---|---|---|
4-NP | 5.50 | 0.79 | 0.30 | 4.41 |
DCF | 5.90 | 0.86 | 0.18 | 4.86 |
EE2 | 5.04 | 0.89 | 1.24 | 2.91 |
None (Control) | 8.64 | 1.78 | 1.96 | 4.90 |
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Olejnik, D.; Galamon, M.; Liwarska-Bizukojc, E.; Delbeke, E.; Van Geem, K.M.; Stevens, C.V. Effect of Newly Synthesized Salts and Three Common Micropollutants on the Biochemical Activity of Nitrifiers. Sustainability 2021, 13, 7417. https://doi.org/10.3390/su13137417
Olejnik D, Galamon M, Liwarska-Bizukojc E, Delbeke E, Van Geem KM, Stevens CV. Effect of Newly Synthesized Salts and Three Common Micropollutants on the Biochemical Activity of Nitrifiers. Sustainability. 2021; 13(13):7417. https://doi.org/10.3390/su13137417
Chicago/Turabian StyleOlejnik, Dorota, Malgorzata Galamon, Ewa Liwarska-Bizukojc, Elisabeth Delbeke, Kevin M. Van Geem, and Christian V. Stevens. 2021. "Effect of Newly Synthesized Salts and Three Common Micropollutants on the Biochemical Activity of Nitrifiers" Sustainability 13, no. 13: 7417. https://doi.org/10.3390/su13137417