Supplemental Effects of Biochar and Foliar Application of Ascorbic Acid on Physio-Biochemical Attributes of Barley (Hordeum vulgare L.) under Cadmium-Contaminated Soil
Abstract
:1. Introduction
2. Materials and Methods
2.1. Seed Sterilization
2.2. Experimental Design
2.3. Seeds Sowing and Pot Preparation
2.4. Cadmium (Cd) Contamination
2.5. Ascorbic Acid (Asa)
2.6. Growth Attributes
2.7. Measurement of Chlorophyll Contents and Gas Exchange Characteristics
2.8. Electrolyte Leakage
2.9. Oxidative Stress Indicators
2.10. Determination of Antioxidant Enzymatic Activities
2.11. Estimation of Proline, Sugars, and Non-Enzymatic Antioxidants
2.12. Analysis of Cadmium Contents
2.13. Statistical Analysis
3. Results
3.1. Root and Shoot Length
3.2. Chlorophyll Contents
3.3. Gas Exchange Attributes
3.4. SOD, POD, and APX
3.5. Catalase, Phenolics, Flavonoids, and Anthocyanin
3.6. Ascorbic Acid, MDA, H2O2, and Electrolyte Leakage
3.7. Proline, Total Soluble Proteins, Root Cd Concentration, and Shoot Cd Concentration
3.8. Pearson Correlation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Yaseen, S.; Amjad, S.F.; Mansoora, N.; Kausar, S.; Shahid, H.; Alamri, S.A.M.; Alrumman, S.A.; Eid, E.M.; Ansari, M.J.; Danish, S.; et al. Supplemental Effects of Biochar and Foliar Application of Ascorbic Acid on Physio-Biochemical Attributes of Barley (Hordeum vulgare L.) under Cadmium-Contaminated Soil. Sustainability 2021, 13, 9128. https://doi.org/10.3390/su13169128
Yaseen S, Amjad SF, Mansoora N, Kausar S, Shahid H, Alamri SAM, Alrumman SA, Eid EM, Ansari MJ, Danish S, et al. Supplemental Effects of Biochar and Foliar Application of Ascorbic Acid on Physio-Biochemical Attributes of Barley (Hordeum vulgare L.) under Cadmium-Contaminated Soil. Sustainability. 2021; 13(16):9128. https://doi.org/10.3390/su13169128
Chicago/Turabian StyleYaseen, Samia, Syeda Fasiha Amjad, Nida Mansoora, Shameem Kausar, Huma Shahid, Saad A. M. Alamri, Sulaiman A. Alrumman, Ebrahem M. Eid, Mohammad Javed Ansari, Subhan Danish, and et al. 2021. "Supplemental Effects of Biochar and Foliar Application of Ascorbic Acid on Physio-Biochemical Attributes of Barley (Hordeum vulgare L.) under Cadmium-Contaminated Soil" Sustainability 13, no. 16: 9128. https://doi.org/10.3390/su13169128