Towards Sustainable Sources of Omega-3 Long-Chain Polyunsaturated Fatty Acids in Northern Australian Tropical Crossbred Beef Steers through Single Nucleotide Polymorphisms in Lipogenic Genes for Meat Eating Quality
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Diets and Experimental Design
2.2. Loin Eye Muscle Sampling and Chemical Analysis
2.3. Blood Sampling and Genomic DNA Extraction
2.4. Primer Design, Amplification of Target Genes, Clean-Up of PCR Products, Library Preparation, Sequencing and Data Analysis
2.5. Calculations and Statistical Analysis
3. Results
3.1. Genetic Diversity of the Identified Single Nucleotide Polymorphisms
3.2. Correlations between Single Nucleotide Polymorphisms, Intramuscular Fat, Fat Melting Point, and Fatty Acid Composition
3.3. Associations between Single Nucleotide Polymorphisms, Intramuscular Fat, Fat Melting Point, and Fatty Acid Composition
4. Discussion
4.1. Fatty Acid Binding Protein 4 Gene Polymorphisms
4.2. Stearoyl-CoA Desaturase Gene Polymorphisms
4.3. Fatty Acid Synthase Gene Polymorphisms
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene 1 | SNP (Variant ID) 2 | PCS Position 4 | Amino Acid Substitution |
---|---|---|---|
FABP4 | g.44677959 T>C (rs110757796) | 220 | Isoleucine to Valine |
SCD | g.21272422 C>T (rs41255693) | 878 | Alanine to Valine |
FASN | g.50782773 G>A (rs715140536) | 1243 | Alanine to Threonine |
g.50784533 C>G (rs481622676) | 2066 | Alanine to Glycine | |
g.50784824 G>A (rs209227647) | 2252 | Arginine to Histidine | |
g.50786496A>G 3 | 3145 | Serine to Glycine | |
g.50788575T>C (rs41919993) | 4168 | Tyrosine to Histidine | |
g.50789448C>T (rs516607144) | 4693 | Leucine to Phenylalanine | |
g.50790973C>A (rs109149276) | 5572 | Leucine to Isoleucine |
Gene/SNP 1 | p-Value 2 | ||||
---|---|---|---|---|---|
FABP4 g.44677239C>G | Total (n = 48) | CC (n = 19) | CG (n = 19) | GG (n = 10) | |
IMF | 2.3 ± 0.75 | 2.1 ± 0.62 | 2.5 ± 0.9 | 2.2 ± 0.67 | 0.38 |
FMP | 43.9 ± 4.79 | 42.7 ± 4.58 | 44.6 ± 4.83 | 44.9 ± 5.13 | 0.53 |
16:0 (Palmitic acid) | 209.1 ± 149.68 | 194.3 ± 113.24 | 238.7 ± 185.99 | 179.5 ± 133.59 | 0.72 |
16:1 (Palmitoleic acid) | 34.6 ± 34.72 | 40.5 ± 47.35 | 34.5 ± 25.6 | 23.3 ± 16.87 | 0.52 |
18:0 (Stearic acid) | 128.6 ± 78.22 | 119.2 ± 66.31 | 139.4 ± 83.68 | 125.1 ± 92.35 | 0.61 |
18:1 (Oleic acid) | 263.1 ± 195.31 | 244.6 ± 143.89 | 302.4 ± 245.96 | 221.4 ± 170.23 | 0.74 |
18:2ω6 (Linoleic acid) | 50.1 ± 10.2 | 45.8 ± 10.88a | 52.0 ± 9.62 ab | 54.5 ± 7.3b | 0.03 |
18:3ω3 (α-linolenic acid) | 16.3 ± 3.14 | 15.7 ± 3.69 | 16.4 ± 2.85 | 16.9 ± 2.73 | 0.73 |
CLA | 4.3 ± 3.33 | 4.2 ± 3.11 | 4.4 ± 3.49 | 4.1 ± 3.77 | 0.79 |
EPA | 9.4 ± 2.18 | 9.2 ± 2.13 | 9.8 ± 2.31 | 9.1 ± 2.12 | 0.58 |
DPA | 14.0 ± 3.37 | 13.0 ± 3.61 | 15.0 ± 2.64 | 13.9 ± 3.92 | 0.12 |
DHA | 2.3 ± 0.8 | 2.3 ± 0.93 | 2.5 ± 0.69 | 2.2 ± 0.79 | 0.28 |
EPA+DHA | 11.8 ± 2.77 | 11.5 ± 2.81 | 12.3 ± 2.76 | 11.4 ± 2.86 | 0.33 |
EPA+DPA+DHA | 25.8 ± 5.79 | 24.6 ± 5.88 | 27.4 ± 5.23 | 25.3 ± 6.53 | 0.14 |
SFA | 376.9 ± 254.18 | 351.5 ± 201.61 | 420.0 ± 298.96 | 340.8 ± 260.83 | 0.75 |
MUFA | 313.4 ± 228.69 | 294.7 ± 173.9 | 358.2 ± 285.22 | 261.9 ± 199.01 | 0.69 |
PUFA | 142.8 ± 26.51 | 134.2 ± 28.71 | 149.6 ± 23.23 | 146.3 ± 25.98 | 0.41 |
ω3 PUFA | 48.2 ± 8.92 | 47.0 ± 10.06 | 49.9 ± 7.84 | 47.5 ± 8.96 | 0.55 |
ω6 PUFA | 79.6 ± 16.18 | 73.0 ± 18.67 | 83.6 ± 13.12 | 84.5 ± 13.17 | 0.11 |
SCD g.21266629 G>T | Total (n = 48) | GG (n = 11) | GT (n = 22) | TT (n = 15) | |
IMF | 2.3 ± 0.75 | 2.2 ± 0.55 | 2.2 ± 0.62 | 2.5 ± 1.03 | 0.64 |
FMP | 43.9 ± 4.79 | 42.9 ± 3.64 | 44.1 ± 6.17 | 44.3 ± 3.26 | 0.78 |
16:0 (Palmitic acid) | 209.1 ± 149.68 | 182.2 ± 111.02 | 201.0 ± 116.16 | 240.2 ± 209.36 | 0.86 |
16:1 (Palmitoleic acid) | 34.6 ± 34.72 | 27.4 ± 16.93 | 38.4 ± 44.8 | 34.2 ± 27.7 | 0.91 |
18:0 (Stearic acid) | 128.6 ± 78.22 | 125.6 ± 76.49 | 122.6 ± 60.52 | 139.2 ± 102.52 | 0.82 |
18:1 (Oleic acid) | 263.1 ± 195.31 | 241.6 ± 164.92 | 255.0 ± 152.87 | 290.1 ± 266.99 | 0.82 |
18:2ω6 (Linoleic acid) | 50.1 ± 10.2 | 50.0 ± 5.35 | 48.0 ± 10.93 | 53.2 ± 11.52 | 0.66 |
18:3ω3 (α-linolenic acid) | 16.3 ± 3.14 | 16.5 ± 2.66 | 15.5 ± 3.24 | 17.1 ± 3.28 | 0.49 |
CLA | 4.3 ± 3.33 | 4.7 ± 4.46 | 4.3 ± 2.71 | 4.0 ± 3.4 | 0.57 |
EPA | 9.4 ± 2.18 | 8.6 ± 1.61 a | 9.2 ± 2.43 ab | 10.3 ± 1.93 b | 0.08 |
DPA | 14.0 ± 3.37 | 12.9 ± 1.78 a | 13.4 ± 3.75 ab | 15.7 ± 3.2 b | 0.03 |
DHA | 2.3 ± 0.8 | 2.1 ± 0.51 a | 2.2 ± 0.88 a | 2.8 ± 0.74 b | 0.02 |
EPA+DHA | 11.8 ± 2.77 | 10.8 ± 1.89 a | 11.4 ± 3.07 ab | 13.1 ± 2.48 b | 0.03 |
EPA+DPA+DHA | 25.8 ± 5.79 | 23.7 ± 3.21 a | 24.8 ± 6.31 ab | 28.9 ± 5.51 b | 0.02 |
SFA | 376.9 ± 254.18 | 345.6 ± 210.17 | 360.6 ± 197.48 | 422.7 ± 348.72 | 0.89 |
MUFA | 313.4 ± 228.69 | 287.2 ± 191.76 | 303.9 ± 181.66 | 345.7 ± 310.88 | 0.85 |
PUFA | 142.8 ± 26.51 | 138.0 ± 15.98 | 137.8 ± 29.03 | 153.7 ± 26.98 | 0.31 |
ω3 PUFA | 48.2 ± 8.92 | 45.5 ± 5.37 | 47.2 ± 10.49 | 51.8 ± 7.77 | 0.12 |
ω6 PUFA | 79.6 ± 16.18 | 78.5 ± 7.52 | 75.9 ± 19.07 | 85.9 ± 15.07 | 0.40 |
FASN g.50783803G>A | Total (n = 48) | GG (n = 20) | GA (n = 20) | AA (n = 8) | |
IMF | 2.3 ± 0.75 | 2.2 ± 0.71 | 2.3 ± 0.60 | 2.5 ± 1.14 | 0.49 |
FMP | 43.9 ± 4.79 | 44.8 ± 3.56 | 43.1 ± 6.01 | 43.8 ± 3.62 | 0.40 |
16:0 (Palmitic acid) | 209.1 ± 149.68 | 161.3 ± 84.44 | 211.4 ± 131.91 | 323.2 ± 248.45 | 0.24 |
16:1 (Palmitoleic acid) | 34.6 ± 34.72 | 26.0 ± 13.60 | 41.8 ± 46.84 | 45.3 ± 31.24 | 0.17 |
18:0 (Stearic acid) | 128.6 ± 78.22 | 103.2 ± 38.01 | 129.8 ± 76.27 | 189.3 ± 123.57 | 0.28 |
18:1 (Oleic acid) | 263.1 ± 195.31 | 196.9 ± 114.57 | 279.5 ± 185.13 | 389.2 ± 309.04 | 0.16 |
18:2ω6 (Linoleic acid) | 50.1 ± 10.20 | 50.5 ± 10.29 | 48.9 ± 9.88 | 52.1 ± 11.72 | 0.95 |
18:3ω3 (α-linolenic acid) | 16.3 ± 3.14 | 16.3 ± 3.00 | 16.1 ± 3.34 | 16.8 ± 3.32 | 0.87 |
CLA | 4.3 ± 3.33 | 3.4 ± 1.70 | 4.8 ± 4.01 | 5.6 ± 4.49 | 0.35 |
EPA | 9.4 ± 2.18 | 9.9 ± 2.25 | 9.0 ± 2.05 | 9.3 ± 2.40 | 0.52 |
DPA | 14.0 ± 3.37 | 14.7 ± 2.78 | 13.1 ± 3.45 | 14.5 ± 4.40 | 0.53 |
DHA | 2.3 ± 0.80 | 2.4 ± 0.78 | 2.3 ± 0.8 | 2.1 ± 0.91 | 0.48 |
EPA+DHA | 11.8 ± 2.77 | 12.3 ± 2.89 | 11.4 ± 2.5 | 11.4 ± 3.25 | 0.44 |
EPA+DPA+DHA | 25.8 ± 5.79 | 27.1 ± 5.48 | 24.6 ± 5.34 | 26.0 ± 7.57 | 0.48 |
SFA | 376.9 ± 254.18 | 294.0 ± 135.9 | 381.0 ± 229.59 | 574.6 ± 417.76 | 0.28 |
MUFA | 313.4 ± 228.69 | 235.3 ± 132.97 | 332.1 ± 217.83 | 464.1 ± 359.61 | 0.20 |
PUFA | 142.8 ± 26.51 | 143.9 ± 22.70 | 139.2 ± 25.97 | 148.9 ± 37.51 | 0.90 |
ω3 PUFA | 48.2 ± 8.92 | 48.9 ± 8.18 | 47.4 ± 9.19 | 48.9 ± 10.91 | 0.82 |
ω6 PUFA | 79.6 ± 16.18 | 81.5 ± 14.45 | 76.8 ± 16.70 | 79.6 ± 19.88 | 0.89 |
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Mwangi, F.W.; Pewan, S.B.; Otto, J.R.; Adegboye, O.A.; Charmley, E.; Gardiner, C.P.; Malau-Aduli, B.S.; Kinobe, R.T.; Malau-Aduli, A.E.O. Towards Sustainable Sources of Omega-3 Long-Chain Polyunsaturated Fatty Acids in Northern Australian Tropical Crossbred Beef Steers through Single Nucleotide Polymorphisms in Lipogenic Genes for Meat Eating Quality. Sustainability 2022, 14, 8409. https://doi.org/10.3390/su14148409
Mwangi FW, Pewan SB, Otto JR, Adegboye OA, Charmley E, Gardiner CP, Malau-Aduli BS, Kinobe RT, Malau-Aduli AEO. Towards Sustainable Sources of Omega-3 Long-Chain Polyunsaturated Fatty Acids in Northern Australian Tropical Crossbred Beef Steers through Single Nucleotide Polymorphisms in Lipogenic Genes for Meat Eating Quality. Sustainability. 2022; 14(14):8409. https://doi.org/10.3390/su14148409
Chicago/Turabian StyleMwangi, Felista W., Shedrach B. Pewan, John R. Otto, Oyelola A. Adegboye, Edward Charmley, Christopher P. Gardiner, Bunmi S. Malau-Aduli, Robert T. Kinobe, and Aduli E. O. Malau-Aduli. 2022. "Towards Sustainable Sources of Omega-3 Long-Chain Polyunsaturated Fatty Acids in Northern Australian Tropical Crossbred Beef Steers through Single Nucleotide Polymorphisms in Lipogenic Genes for Meat Eating Quality" Sustainability 14, no. 14: 8409. https://doi.org/10.3390/su14148409
APA StyleMwangi, F. W., Pewan, S. B., Otto, J. R., Adegboye, O. A., Charmley, E., Gardiner, C. P., Malau-Aduli, B. S., Kinobe, R. T., & Malau-Aduli, A. E. O. (2022). Towards Sustainable Sources of Omega-3 Long-Chain Polyunsaturated Fatty Acids in Northern Australian Tropical Crossbred Beef Steers through Single Nucleotide Polymorphisms in Lipogenic Genes for Meat Eating Quality. Sustainability, 14(14), 8409. https://doi.org/10.3390/su14148409