Sustainable Environmental-Based ZnO Nanoparticles Derived from Pisonia grandis for Future Biological and Environmental Applications
Round 1
Reviewer 1 Report
Reviewing report: “Sustainable Environmental-Based ZnO Nanoparticles …”
In this work, the authors tried to synthesize and characterize zinc oxide nanoparticles using a plant species. Although the subject of this research can be interesting, but there are some issues that need to be answered accurately.
1. It is suggested to include some information about Pisonia grandis in the Introduction of the article. Meantime, the reason why it is used for synthesis, should be included.
2. The relevance/novelty of the work needs to be highlighted.
3. How and in what way the authors were able to obtain a temperature of 150 C in the reaction?
4. It is suggested to replace the term “fabrication” by “synthesis”.
5. Page 3, what is meant by blasted powder?
6. The conditions of XRD such as rate should be stated.
7. Page 5, what is meant by “ZnO nanoparticles therapy”?
8. What is meant by “…ZnO nanoparticles synthesized by P. 167 grandis at 2 were found…”?
9. Page 5, line 138, there are no peaks at two theta stated.
10. Page 5, line 174, how the authors determined the crystallite size by Scherer's method. The details should be stated.
11. Presenting the XRD pattern of zinc oxide product in case of not using the plant can highlight the importance of this type of plant.
12. What exactly does FTIR-analysis show in this work and for what purpose was it done? It should be mentioned exactly from which stage in the synthesis, FTIR spectrum was obtained.
13. Are the particles really semispherical? They seem to be flat. The word “cluster” should be replaced with “agglomerate”.
14. Do the authors think that the “particle size” is the same as the “crystallite size”?
15. How have the authors been able to distinguish the particle size range from 20 to 40 from the Figure 4?
16. In Figure 5, is the information with the TEM micrograph correct? Pay attention to bar scale and magnification.
17. In antibacterial test, it should be included an image of medium and diameter of specimens in bacteria medium.
18. Page 9, lines 257-262, is the interpretation provided correct? Note that after synthesis and heating at high temperature, the substances in the plant are not present in the sample.
19. Based on the points of view given above, it is suggested to rewrite the conclusion of the article.
Comments for author File: 
Comments.pdf
Author Response
As per your suggestion has been carried out.
Author Response File: Author Response.pdf
Reviewer 2 Report
The work by Kaliyamoorthy et al. presents an interesting study about green synthesized ZnO nanoparticles derived from Pisonia grandis for biological and environmental applications. The work includes a detailed discussion and multiple characterization techniques to support the conclusion. The work can be accepted for publication in the journal Sustainability after the necessary corrections (yellow color) indicated within the text were done.
Comments for author File: Comments.pdf
Author Response
As per your suggestion has been carried out.
Author Response File: Author Response.pdf
Reviewer 3 Report
I want to give following commends after revising the Manuscript.
§ In materials and method section, the author should describe the grade and specification of materials that you used in this research
§ Which part of plant you used for extraction?
§ The author should reveal the condition of the parts of the plant, such as dried or fresh.
§ When the parts of the plant calcining at 500 for 2h, can you confirm the stability of active constituents of the plant
§ Why do you use two different temperatures for culturing the organisms?
§ In section 2.4.1, do you inject 20L of substance? It is so surprise.
§ When you kept (+) and (-) control, the author should mention that which one is (+) and which one is (-).
§ Why don’t you use 96 well plates for MTT assay?
§ In photodegradation evaluation, the author should mention the equation to calculate the percentage deterioration of the degraded dye.
§ In figure 6, please put the legend. Why were the zone of inhibition not constant in control?
§ In figure 8, please keep unit for absorption.
Author Response
As per your suggestion has been carried out.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
The new version of the article does not need to be modified.Reviewer 3 Report
Well receive your great response.
