The Recovery of a Sequencing Biofilm Batch Reactor—Anammox System: Performance, Metabolic Characteristics, and Microbial Community Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Set-Up
2.2. Substrate Composition and System Properties Analysis
2.3. Extracellular Polymeric Substances (EPS) Content
2.4. Metabolic Enzyme and Electron Transport System Activities
2.5. 16S rRNA Amplicon Sequencing
3. Results and Discussion
3.1. SBBR–Anammox System Performance
3.2. Variation in Metabolic Characteristics
3.2.1. Changes of EPS Contents
3.2.2. Metabolic Enzyme Activity
3.3. Microbial Community Analysis
3.3.1. Microbial Community Dynamics
3.3.2. Microbial Community Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Period | Water Inlet | Anaerobic Reaction | Sedimentation | Water Outlet | Idle |
---|---|---|---|---|---|
Time | 15 min | 360 min | 80 min | 15 min | 10 min |
Samples | Reads | OTU (97%) | Shannon Index | Simpson Index |
---|---|---|---|---|
Stage I | 40,582.0 | 425.0 | 3.09 | 0.17 |
Stage II | 48,778.0 | 487.0 | 3.45 | 0.11 |
Stage III | 63,248.0 | 508.0 | 3.44 | 0.12 |
Stage IV | 21,299.0 | 414.0 | 3.60 | 0.08 |
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Chen, X.; Liu, L.; Bi, Y.; Meng, F.; Wang, D.; Qiu, C.; Yu, J.; Wang, S. The Recovery of a Sequencing Biofilm Batch Reactor—Anammox System: Performance, Metabolic Characteristics, and Microbial Community Analysis. Sustainability 2023, 15, 10454. https://doi.org/10.3390/su151310454
Chen X, Liu L, Bi Y, Meng F, Wang D, Qiu C, Yu J, Wang S. The Recovery of a Sequencing Biofilm Batch Reactor—Anammox System: Performance, Metabolic Characteristics, and Microbial Community Analysis. Sustainability. 2023; 15(13):10454. https://doi.org/10.3390/su151310454
Chicago/Turabian StyleChen, Xiaoying, Lingjie Liu, Yanmeng Bi, Fansheng Meng, Dong Wang, Chunsheng Qiu, Jingjie Yu, and Shaopo Wang. 2023. "The Recovery of a Sequencing Biofilm Batch Reactor—Anammox System: Performance, Metabolic Characteristics, and Microbial Community Analysis" Sustainability 15, no. 13: 10454. https://doi.org/10.3390/su151310454