Skip to Content

205 Results Found

  • Article
  • Open Access
23 Citations
7,193 Views
11 Pages

Characterization of a Microbial Community in an Anammox Process Using Stored Anammox Sludge

  • Wenjing Chen,
  • Xiaohu Dai,
  • Dawen Cao,
  • Xiaona Hu,
  • Wenru Liu and
  • Dianhai Yang

29 October 2017

This study investigated a rapid start-up anaerobic ammonium oxidation (Anammox) process by inoculation with stored Anammox sludge and characterized the associated microbial communities. The Anammox process took only 43 days to start. A high nitrogen...

  • Article
  • Open Access
91 Citations
9,806 Views
13 Pages

Cometabolism of the Superphylum Patescibacteria with Anammox Bacteria in a Long-Term Freshwater Anammox Column Reactor

  • Suguru Hosokawa,
  • Kyohei Kuroda,
  • Takashi Narihiro,
  • Yoshiteru Aoi,
  • Noriatsu Ozaki,
  • Akiyoshi Ohashi and
  • Tomonori Kindaichi

16 January 2021

Although the anaerobic ammonium oxidation (anammox) process has attracted attention regarding its application in ammonia wastewater treatment based on its efficiency, the physiological characteristics of anammox bacteria remain unclear because of the...

  • Article
  • Open Access
4 Citations
3,481 Views
15 Pages

8 August 2023

Anaerobic ammonia oxidation bacteria (AnAOB) are difficult to cultivate due to their long start-up time and sensitivity to environmental conditions. In this study, anammox granular sludge was cultured with ordinary activated sludge under influent dis...

  • Review
  • Open Access
25 Citations
9,249 Views
21 Pages

Advances in the Efficient Enrichment of Anammox Bacteria

  • Yuting Fu,
  • Xin Wen,
  • Jiansheng Huang,
  • Da Sun and
  • Libo Jin

12 July 2023

Anaerobic ammonia oxidation (anammox) process is known as a low-energy and environmentally friendly process for treating nitrogen-rich wastewater. Anammox bacteria are the key microorganisms to achieve this biological process. However, the efficient...

  • Article
  • Open Access
2,025 Views
14 Pages

Anaerobic ammonium oxidation (anammox) is a key process in the removal of nitrogen from wastewater, in which episodes of substrate inhibition may occur. In this study, the effect of nitrite on anammox in the short and long term was investigated using...

  • Article
  • Open Access
1 Citations
2,118 Views
12 Pages

Evidence of Potential Anammox Activities from Rice Paddy Soils in Microaerobic and Anaerobic Conditions

  • Anamika Khanal,
  • Hyung-Geun Song,
  • Yu-Sung Cho,
  • Seo-Yeon Yang,
  • Won-Seok Kim,
  • Alpana Joshi,
  • Jiho Min and
  • Ji-Hoon Lee

19 July 2024

Anammox, a reaction in which microorganisms oxidize ammonia under anaerobic conditions, is used in the industry to remove ammonium from wastewater in an environmentally friendly manner. This process does not produce intermediate products such as nitr...

  • Article
  • Open Access
6 Citations
2,620 Views
14 Pages

The long multiplication time and extremely demanding enrichment environment requirements of Anammox bacteria (AAOB) have led to difficult reactor start-ups and hindered its practical dissemination. Few feasibility studies have been reported on the re...

  • Article
  • Open Access
2 Citations
2,890 Views
15 Pages

8 February 2024

Removal of fixed nitrogen compounds such as ammonium and nitrite from wastewater is of critical importance for balancing the nitrogen cycle and protecting aquatic environments from eutrophication. ANaerobic AMMonium OXidising (ANAMMOX) bacteria have...

  • Article
  • Open Access
5 Citations
2,475 Views
19 Pages

25 March 2024

The Anammox anaerobic fluidized bed microbial fuel cell (Anammox AFB-MFC) exhibits exceptional performance in both nitrogen removal and electricity generation, effectively eliminating ammonia nitrogen (NH4+-N) and nitrite nitrogen (NO2-N) poll...

  • Feature Paper
  • Article
  • Open Access
77 Citations
7,566 Views
16 Pages

Start-Up of Anammox SBR from Non-Specific Inoculum and Process Acceleration Methods by Hydrazine

  • Ivar Zekker,
  • Oleg Artemchuk,
  • Ergo Rikmann,
  • Kelvin Ohimai,
  • Gourav Dhar Bhowmick,
  • Makarand Madhao Ghangrekar,
  • Juris Burlakovs and
  • Taavo Tenno

30 January 2021

Biological nutrient removal from wastewater to reach acceptable levels is needed to protect water resources and avoid eutrophication. The start-up of an anaerobic ammonium oxidation (anammox) process from scratch was investigated in a 20 L sequence b...

  • Article
  • Open Access
12 Citations
3,855 Views
22 Pages

29 September 2022

The complex pollution of aquifers by reduced and oxidized nitrogen compounds is currently considered one of the urgent environmental problems that require non-standard solutions. This work was a laboratory-scale trial to show the feasibility of using...

  • Article
  • Open Access
3 Citations
2,877 Views
14 Pages

Effect of Sulfide on the Processes of Transformation of Nitrogen Compounds and the Microbial Community Composition in the Anammox Bioreactor

  • Nikolai Pimenov,
  • Yuri Nikolaev,
  • Vladimir Grachev,
  • Anna Kallistova,
  • Alexander Dorofeev,
  • Yuriy Litti,
  • Evgeny Gruzdev,
  • Alexey Beletsky,
  • Nikolai Ravin and
  • Andrey Mardanov

2 August 2023

Anammox is one of the most important processes in the global nitrogen cycle and the basis for an efficient technology of nitrogen removal from wastewater. The effect of the presence of sulfide in wastewater on the transformation of nitrogen compounds...

  • Article
  • Open Access
8 Citations
3,953 Views
8 Pages

A Simple Analysis Method of Specific Anammox Activity Using a Respirometer

  • Jaecheul Yu,
  • Yeonju Kim,
  • Jeongmi Kim,
  • Soyeon Jeong,
  • Seongjae Park and
  • Taeho Lee

21 January 2022

Anaerobic ammonium oxidation (anammox) is a biological nitrogen removal process with attractive prospects, such as no carbon addition, less aeration, lower greenhouse gas generation, and lower sludge production. However, it is difficult to maintain a...

  • Article
  • Open Access
6 Citations
3,325 Views
10 Pages

Application of the Anammox Process for Treatment of Liquid Phase Digestate

  • Joanna Majtacz,
  • Dominika Grubba and
  • Krzysztof Czerwionka

22 October 2020

The liquid phase of the digestate (LPD) contains a relatively high concentration of nitrogen, with total ammonium nitrogen being the dominant form of nitrogen, as well as other essential nutrients such as phosphorus and potassium. Consequently, it mu...

  • Article
  • Open Access
1 Citations
5,027 Views
14 Pages

Effects of Caffeine and COD from Coffee Wastewater on Anaerobic Ammonium Oxidation (Anammox) Activities

  • Titima Wongphoom,
  • Tharinee Saleepochn,
  • Pongsak Lek Noophan and
  • Chi-Wang Li

16 July 2022

An anaerobic ammonium oxidation (anammox) process was employed to remove nitrogen from wastewater generated from a coffee brewing facility. The effects of caffeine and chemical oxygen demand (COD) in coffee wastewater on anammox activity were investi...

  • Article
  • Open Access
7 Citations
5,371 Views
12 Pages

Achieving Partial Nitritation in Anammox Start-Up Environment

  • Sabin Pathak,
  • Shuai Wang and
  • Eshetu Janka

13 January 2022

Removing ammonium via the partial nitritation anammox (PNA) process has been widely applied because of its cost and energy effectiveness. However, the first stage of PNA, partial nitritation, is hard to implement practically due to the challenging su...

  • Article
  • Open Access
1 Citations
2,580 Views
11 Pages

11 May 2023

Free anammox bacteria are superior in growth rate, but poor sedimentation performance limits their application in sewage treatment. In this study, we investigated how to form aggregates of free anammox bacteria to improve sedimentation performance. C...

  • Communication
  • Open Access
9 Citations
3,193 Views
10 Pages

The Development of Anammox and Chloroflexi Bacteria during the Composting of Sewage Sludge

  • Sylwia Stegenta-Dąbrowska,
  • Karolina Sobieraj,
  • Joanna Rosik,
  • Robert Sidełko,
  • Marvin Valentin and
  • Andrzej Białowiec

18 August 2022

The C/N ratio is an extremely important parameter in the composting process and is directly responsible for the growth of microorganisms. A low C/N ratio contributes to higher emissions of greenhouse gases and odorous substances, such as ammonia (NH3...

  • Feature Paper
  • Review
  • Open Access
37 Citations
12,379 Views
15 Pages

20 November 2017

Water quality in San Francisco Bay has been adversely affected by nitrogen loading from wastewater treatment plants (WWTPs) discharging around the periphery of the Bay. While there is documented use of zeolites and anammox bacteria in removing ammoni...

  • Review
  • Open Access
5 Citations
2,766 Views
13 Pages

3 February 2023

Anaerobic ammonium oxidation (anammox) has shown success in past years for the treatment of municipal and industrial wastewater containing inorganic nutrients (i.e., nitrogen). However, the increase in polycyclic aromatic hydrocarbon (PAH)-contaminat...

  • Feature Paper
  • Article
  • Open Access
15 Citations
4,545 Views
12 Pages

25 February 2018

Water quality in San Francisco Bay is reportedly adversely affected by nitrogen loading from the wastewater treatment plants (WWTPs) discharging around the periphery of the Bay. Here, we consider a zeolite-anammox system to remove ammonia and nitrate...

  • Review
  • Open Access
13 Citations
4,237 Views
16 Pages

Harnessing the Potential of Extracellular Polymeric Substances in Enhancing ANAMMOX Processes: Mechanisms, Strategies, and Perspectives

  • Lijing Fan,
  • Cancan Jiang,
  • Xu Wang,
  • Yang Yang,
  • Yawen Xie,
  • Jiaqi Su,
  • Hong Sun,
  • Shengjun Xu and
  • Xuliang Zhuang

26 April 2024

Anaerobic ammonium oxidation (ANAMMOX) has emerged as a promising sustainable nitrogen removal technology that offers significant advantages over conventional nitrification–denitrification processes, such as reduced energy consumption, a 60% re...

  • Article
  • Open Access
26 Citations
5,002 Views
14 Pages

A one-stage anaerobic ammonium oxidation (ANAMMOX) reactor can be quickly started within 40 days by mixing partial nitrifying sludge with ANAMMOX granular sludge with an average temperature of 30 °C. After 70 days of nitrogen load acclimation, Ac...

  • Review
  • Open Access
21 Citations
6,191 Views
14 Pages

28 June 2022

Wastewater treatment plants (WWTPs) are important contributors to global greenhouse gas (GHG) emissions, partly due to their huge emission of nitrous oxide (N2O), which has a global warming potential of 298 CO2 equivalents. Anaerobic ammonium-oxidizi...

  • Article
  • Open Access
13 Citations
5,794 Views
12 Pages

Effects of Recirculating Aquaculture System Wastewater on Anammox Performance and Community Structure

  • Jonathan A. C. Roques,
  • Federico Micolucci,
  • Suguru Hosokawa,
  • Kristina Sundell and
  • Tomonori Kindaichi

7 July 2021

Recirculating aquaculture systems (RAS) are good candidates for the sustainable development of the aquaculture sector. A current limitation of RAS is the production and accumulation of nitrogenous waste, which could affect fish health. We investigate...

  • Feature Paper
  • Article
  • Open Access
16 Citations
4,507 Views
12 Pages

1 November 2018

State regulatory and other agencies identified that nitrogen loading from the wastewater treatment plants (WWTPs) discharging around its periphery has adversely affected the San Francisco Bay (SFB) water quality. Here we consider the upscaling of the...

  • Article
  • Open Access
3 Citations
3,336 Views
13 Pages

Effect of Fe2+ on ANAMMOX Granular Sludge Cultured in a Biased Acidic Influent and Dynamic Environment

  • Kunming Fu,
  • Yihao Bian,
  • Shan Jiang,
  • Sibo Fu,
  • Jia Kang,
  • Xiaodan Li,
  • Zirui Li and
  • Wenbing Yang

27 October 2023

A continuous stirred tank reactor (CSTR) was utilized in this study to enrich and cultivate anaerobic ammonia oxidation process (ANAMMOX) granular sludge by gradually decreasing its pH, and to investigate the effects of different concentrations of fe...

  • Review
  • Open Access
4 Citations
2,218 Views
18 Pages

10 November 2025

Wastewater nitrogen pollution is a serious environmental problem, and traditional treatment techniques are frequently constrained by their high energy requirements and operational complexity. The anaerobic ammonium oxidation (anammox) process combine...

  • Article
  • Open Access
1 Citations
1,201 Views
19 Pages

14 February 2025

Soil anaerobic ammonium oxidation (anammox) can eliminate reactive nitrogen (N) without generating nitrous oxide and is a key factor in N loss in agricultural ecosystems. Nevertheless, it remains unclear what determines the anammox rate and hzs gene...

  • Article
  • Open Access
16 Citations
4,159 Views
15 Pages

Differences in the Effects of Calcium and Magnesium Ions on the Anammox Granular Properties to Alleviate Salinity Stress

  • Yeonju Kim,
  • Jaecheul Yu,
  • Soyeon Jeong,
  • Jeongmi Kim,
  • Seongjae Park,
  • Hyokwan Bae,
  • Sung-Keun Rhee,
  • Tatsuya Unno,
  • Shou-Qing Ni and
  • Taeho Lee

21 December 2021

Divalent cations were known to alleviate salinity stress on anammox bacteria. Understanding the mechanism of reducing the salinity stress on anammox granules is essential for the application of the anammox process for saline wastewater treatment. In...

  • Article
  • Open Access
1,035 Views
20 Pages

14 July 2025

Nanotechnology plays an increasingly important role in the economy and human life, which means that more and more amounts of nanosubstances, including nanoparticles of metal oxides, together with wastewater, end up in the environment. This study aime...

  • Article
  • Open Access
5 Citations
1,831 Views
13 Pages

Significance of Influent C/N Ratios in Mainstream Anammox Process: Nitrogen Removal and Microbial Dynamics

  • Yandong Yang,
  • Shichong Liu,
  • Lei Liu,
  • Yanan Long,
  • Chao Wang and
  • Changqing Liu

15 February 2025

Achieving simultaneous anammox and denitrification is a feasible approach for enhancing nitrogen removal in mainstream anammox processes. Nevertheless, the optimal C/N range and microbial dynamics driving this process are still not fully understood....

  • Review
  • Open Access
169 Citations
19,570 Views
30 Pages

19 December 2019

The anaerobic ammonium oxidation (anammox) process is well-known as a low-energy consuming and eco-friendly technology for treating nitrogen-rich wastewater. Although the anammox reaction was widely investigated in terms of its application in many wa...

  • Article
  • Open Access
3 Citations
2,111 Views
14 Pages

10 July 2024

Anammox bacteria were embedded with different mass fractions of polyvinyl alcohol (PVA), polyethylene glycol (PEG) and water-based polyurethane (WPU) materials. The embedded immobilized pellets with different particle sizes of about 2.8–3.2 mm were p...

  • Article
  • Open Access
347 Views
14 Pages

Effects of Microplastics on Nitrogen Removal Performance of Enriched Anammox Cultures

  • Yanisa Khotchachain,
  • Tharinee Saleepochn,
  • Pongsak (Lek) Noophan and
  • Chi-Wang Li

30 January 2026

Microplastics (MPs) and their chemical leachates are increasingly detected in landfill leachate, raising concerns about impacts on biological nitrogen removal. This study examined the effects of low-density polyethylene (LDPE) and polypropylene (PP)...

  • Article
  • Open Access
6 Citations
2,695 Views
14 Pages

16 December 2024

Anammox has emerged as a primary alternative to conventional biological nitrogen removal because of its excellent nitrogen removal performance and minimal energy utilization. However, the slow growth and reproduction of anammox bacteria (AnAOB) leads...

  • Article
  • Open Access
10 Citations
2,796 Views
13 Pages

26 July 2024

To expedite enrichment of anaerobic ammonia-oxidizing bacteria (AnAOB) as a way to reduce the start-up time, leading to a quicker transition into stable operation, the anaerobic ammonia oxidation (anammox) process was initiated by a biofilm reactor w...

  • Feature Paper
  • Article
  • Open Access
7 Citations
3,333 Views
12 Pages

An Analysis of Operation Conditions and Microbial Characteristics in Swine Wastewater Treatment Plants with Spontaneously Enriched Anammox Bacteria

  • Miyoko Waki,
  • Chikako Ishimoto,
  • Ryu Suto,
  • Takafumi Nagamine,
  • Toshimi Matsumoto,
  • Hirohide Uenishi,
  • Tomoko Yasuda and
  • Yasuyuki Fukumoto

7 June 2021

The spontaneous enrichment of anammox bacteria has been reported in swine wastewater treatment facilities. However, their causative conditions and microbial characteristics, which this study aims to explain, are poorly understood. We discovered eight...

  • Article
  • Open Access
26 Citations
7,145 Views
16 Pages

21 February 2019

Anammox-based processes have been widely applied for the treatment of wastewater (e.g., wastewater irrigation systems and constructed wetland) which consists of bio-refractory humic substances. Nonetheless, the impacts of bio-refractory humic substan...

  • Article
  • Open Access
6 Citations
2,599 Views
16 Pages

The Recovery of a Sequencing Biofilm Batch Reactor—Anammox System: Performance, Metabolic Characteristics, and Microbial Community Analysis

  • Xiaoying Chen,
  • Lingjie Liu,
  • Yanmeng Bi,
  • Fansheng Meng,
  • Dong Wang,
  • Chunsheng Qiu,
  • Jingjie Yu and
  • Shaopo Wang

3 July 2023

The mainstream application of the anammox process is often hindered by its long recovery phase and instability under disturbance. In this study, a lab-scale anammox sequencing biofilm batch reactor (SBBR) was rapidly recovered within 85 days by gradu...

  • Article
  • Open Access
54 Citations
10,785 Views
16 Pages

Three-Dimensional Stratification of Bacterial Biofilm Populations in a Moving Bed Biofilm Reactor for Nitritation-Anammox

  • Robert Almstrand,
  • Frank Persson,
  • Holger Daims,
  • Maria Ekenberg,
  • Magnus Christensson,
  • Britt-Marie Wilén,
  • Fred Sörensson and
  • Malte Hermansson

29 January 2014

Moving bed biofilm reactors (MBBRs) are increasingly used for nitrogen removal with nitritation-anaerobic ammonium oxidation (anammox) processes in wastewater treatment. Carriers provide protected surfaces where ammonia oxidizing bacteria (AOB) and a...

  • Article
  • Open Access
6 Citations
3,468 Views
13 Pages

Nutrient removal in conventional wastewater treatment systems is expensive due to the high aeration costs. An alternative method for effective and sustainable nitrogen removal in wastewater treatment is anaerobic ammonium oxidation (Anammox) implemen...

  • Article
  • Open Access
590 Views
19 Pages

Anammox is a promising approach for biological nitrogen removal, but the differences in microbial community structure across different systems and their response mechanisms to environmental factors remain unclear. In this study, 206 microbial samples...

  • Article
  • Open Access
1 Citations
942 Views
18 Pages

Fe2+-Coupled Organic-Substrate-Enhanced Nitrogen Removal in Two-Stage Anammox Biofilm Reactors

  • Yingchun Bao,
  • Qilong Ge,
  • Siyuan Li,
  • Xiaowei Wang,
  • Xuwen Zheng and
  • Zhenguo Chen

21 May 2025

Anammox is a novel and energy-efficient biological nitrogen removal technology. Enhancing its performance in treating low-strength nitrogen wastewater is essential for expanding its practical applications. In response to challenges such as low nitrog...

  • Article
  • Open Access
14 Citations
5,032 Views
25 Pages

Characterization of Enrichment Cultures of Anammox, Nitrifying and Denitrifying Bacteria Obtained from a Cold, Heavily Nitrogen-Polluted Aquifer

  • Ekaterina Botchkova,
  • Anastasia Vishnyakova,
  • Nadezhda Popova,
  • Marina Sukhacheva,
  • Tatyana Kolganova,
  • Yuriy Litti and
  • Alexey Safonov

30 January 2023

Anammox bacteria related to Candidatus Scalindua were recently discovered in a cold (7.5 °C) aquifer near sludge repositories containing solid wastes of uranium and processed polymetallic concentrate. Groundwater has a very high level of nitrate...

  • Article
  • Open Access
4 Citations
2,953 Views
13 Pages

13 May 2020

Fe-dependent nitrate reduction by anammox consortia could serve as a valuable autotrophic denitrification process for wastewater treatment. However, influences of temperature, pH, and Fe/NO3-N ratio on this biochemical process have not been studied....

  • Article
  • Open Access
637 Views
20 Pages

Reactivation and Nitrogen Removal Performance of Idle Anammox Sludge Enhanced by Rape Straw Biochar

  • Qiang Chen,
  • Yi Ding,
  • Zhicheng Xu,
  • Haibin Zhou,
  • Ruoyu Zhang,
  • Jiao Chen,
  • Yixin Lu and
  • Wenlai Xu

20 December 2025

Low reactivation efficiency of idle anaerobic ammonia oxidation (anammox) sludge hinders its reapplication. To address this issue, rape straw biochar (RSB) was added in the reactivation process of idle anammox sludge, and its effects on the nitrogen...

  • Article
  • Open Access
14 Citations
3,179 Views
18 Pages

24 September 2020

A tremendous input of ammonium and rare earth elements (REEs) has entered the surroundings on account of the discharge and leak of leaching agents during rare earth in-suit leaching mining, which has threatened various organisms. Anammox has the pote...

  • Review
  • Open Access
80 Views
19 Pages

Partial Sulfur-Driven Denitrification: A Promising Pathway to Break Through the Nitrite Bottleneck in the Anammox Process

  • Tiancheng Yang,
  • Xu Wang,
  • Yang Yang,
  • Yawen Xie,
  • Xinyuan Zhang,
  • Yunxiang Zhang,
  • Yuhan Ge,
  • Cancan Jiang and
  • Xuliang Zhuang

13 March 2026

The anammox technology, as an efficient and energy-saving denitrification method, has been widely used in the field of wastewater treatment. Nevertheless, this process faces two key challenges in actual operation, namely the fluctuation of nitrite su...

  • Proceeding Paper
  • Open Access
1 Citations
2,456 Views
9 Pages

Short-Term and Long-Term Inhibitory Effects of Copper on Anammox Process

  • Cigdem Kalkan Aktan,
  • Ayse Ekin Uzunhasanoglu,
  • Kozet Yapsakli and
  • Bulent Mertoglu

The main goal of this study is to evaluate the short-term and long-term inhibitory effects of Cu (II) on Anammox process. To investigate the short-term inhibition level, four different concentrations (1, 2.5, 5 and 10 mg L−1 as Cu2+) were tested in b...

of 5