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Sustainability
Volume 16
Issue 20
10.3390/su16208946
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Article

In Vivo Study of the Effect of Sugarcane Bagasse Lignin Supplementation on Broiler Chicken Diet as a Step to Validate the Established Chicken Gastrointestinal Tract In Vitro Model

by
Nelson Mota de Carvalho
1,
Carla Giselly de Souza
1,
Célia Maria Costa
1,
Cláudia Castro
1,
Joana F. Fangueiro
1,
Bruno Horta
1,
Divanildo Outor-Monteiro
2,3,4,
José Teixeira
2,
José Luís Mourão
2,3,4,
Victor Pinheiro
2,3,4,
Ana L. Amaro
1,
Patrícia Santos Costa
1,
Catarina S. S. Oliveira
1,
Manuela Estevez Pintado
1,
Diana Luazi Oliveira
1 and
Ana Raquel Madureira
1,*
1
CBQF—Centro de Biotecnologia e Química Fina—Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Diogo Botelho 1327, 4169-005 Porto, Portugal
2
Department Animal Science, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal
3
Animal and Veterinary Research Centre (CECAV), University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal
4
Al4Animals, Department of Animal Science, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal
*
Author to whom correspondence should be addressed.
Sustainability 2024, 16(20), 8946; https://doi.org/10.3390/su16208946
Submission received: 4 September 2024 / Revised: 2 October 2024 / Accepted: 10 October 2024 / Published: 16 October 2024
(This article belongs to the Special Issue By-Products of the Agri-Food Industry: Use for Food Fortification)
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Abstract

Since the global restrictions on antibiotics in poultry systems, there has been a growing demand for natural and sustainable feed additives for disease prevention and poultry nutrition. This study evaluated the effects of incorporating sugarcane bagasse (SCB) lignin into broiler chicken diets. The performance of the chickens, including body weight, feed intake, and mortality, as well as intestinal histomorphometry, and cecum content pH, microbiota, and volatile fatty acids were assessed. In addition, we also aimed to validate an in vitro gastrointestinal tract (GIT) model developed by Carvalho et al. (2023). One hundred and eight 1-day-old Ross 308 chicks were randomly and equally divided into two groups. The first group was fed a basal diet (BD group), while the second group was fed a basal diet supplemented with 1% (w/w) SCB lignin (BD + SCB lignin group) for 36 days. The in vivo conditions of the chicken GIT were replicated in an in vitro model. In the in vivo study, SCB lignin increased cecum acetate and butyrate levels while reducing Bifidobacterium and Enterobacteriaceae, without affecting productivity (body weight, feed intake, and mortality). The in vitro assessment reflected microbiota trends observed in vivo, although without statistical significance. The divergence in organic acid production between the in vivo and in vitro conditions likely resulted from issues with inoculum preparation. This study demonstrates that SCB lignin incorporation positively influences cecal microbiota composition without impacting the animals’ productivity and physiology, suggesting its potential as a functional feed additive. For a more reliable in vitro model, adjustments in inoculum preparation are necessary.
Keywords: chicken; cecal microbiota; fermentation; in vivo; in vitro model; organic acids chicken; cecal microbiota; fermentation; in vivo; in vitro model; organic acids
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MDPI and ACS Style

de Carvalho, N.M.; Souza, C.G.d.; Costa, C.M.; Castro, C.; Fangueiro, J.F.; Horta, B.; Outor-Monteiro, D.; Teixeira, J.; Mourão, J.L.; Pinheiro, V.; et al. In Vivo Study of the Effect of Sugarcane Bagasse Lignin Supplementation on Broiler Chicken Diet as a Step to Validate the Established Chicken Gastrointestinal Tract In Vitro Model. Sustainability 2024, 16, 8946. https://doi.org/10.3390/su16208946

AMA Style

de Carvalho NM, Souza CGd, Costa CM, Castro C, Fangueiro JF, Horta B, Outor-Monteiro D, Teixeira J, Mourão JL, Pinheiro V, et al. In Vivo Study of the Effect of Sugarcane Bagasse Lignin Supplementation on Broiler Chicken Diet as a Step to Validate the Established Chicken Gastrointestinal Tract In Vitro Model. Sustainability. 2024; 16(20):8946. https://doi.org/10.3390/su16208946

Chicago/Turabian Style

de Carvalho, Nelson Mota, Carla Giselly de Souza, Célia Maria Costa, Cláudia Castro, Joana F. Fangueiro, Bruno Horta, Divanildo Outor-Monteiro, José Teixeira, José Luís Mourão, Victor Pinheiro, and et al. 2024. "In Vivo Study of the Effect of Sugarcane Bagasse Lignin Supplementation on Broiler Chicken Diet as a Step to Validate the Established Chicken Gastrointestinal Tract In Vitro Model" Sustainability 16, no. 20: 8946. https://doi.org/10.3390/su16208946

APA Style

de Carvalho, N. M., Souza, C. G. d., Costa, C. M., Castro, C., Fangueiro, J. F., Horta, B., Outor-Monteiro, D., Teixeira, J., Mourão, J. L., Pinheiro, V., Amaro, A. L., Costa, P. S., Oliveira, C. S. S., Pintado, M. E., Oliveira, D. L., & Madureira, A. R. (2024). In Vivo Study of the Effect of Sugarcane Bagasse Lignin Supplementation on Broiler Chicken Diet as a Step to Validate the Established Chicken Gastrointestinal Tract In Vitro Model. Sustainability, 16(20), 8946. https://doi.org/10.3390/su16208946

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