Further Evidence on Efficacy of Diet Supplementation with Fatty Acids in Ocular Pathologies: Insights from the EAE Model of Optic Neuritis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Induction of EAE
2.3. Dietary Supplementation
2.4. Isolation of Total RNA and Proteins
2.5. Quantitative Real Time PCR
2.6. Western Blot
2.7. Electron Microscopy and Quantitative Analysis
2.8. Electroretinography
2.9. Statistical Analysis
3. Results
3.1. Supplementation with FAs Shifts M1 Macrophages toward M2 Phenotype
3.2. Supplementation with FAs Counteracts Optic Nerve Damage
3.3. Supplementation with FAs Prevents the Reduction of PhNR Amplitude
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Locri, F.; Cammalleri, M.; Pini, A.; Dal Monte, M.; Rusciano, D.; Bagnoli, P. Further Evidence on Efficacy of Diet Supplementation with Fatty Acids in Ocular Pathologies: Insights from the EAE Model of Optic Neuritis. Nutrients 2018, 10, 1447. https://doi.org/10.3390/nu10101447
Locri F, Cammalleri M, Pini A, Dal Monte M, Rusciano D, Bagnoli P. Further Evidence on Efficacy of Diet Supplementation with Fatty Acids in Ocular Pathologies: Insights from the EAE Model of Optic Neuritis. Nutrients. 2018; 10(10):1447. https://doi.org/10.3390/nu10101447
Chicago/Turabian StyleLocri, Filippo, Maurizio Cammalleri, Alessandro Pini, Massimo Dal Monte, Dario Rusciano, and Paola Bagnoli. 2018. "Further Evidence on Efficacy of Diet Supplementation with Fatty Acids in Ocular Pathologies: Insights from the EAE Model of Optic Neuritis" Nutrients 10, no. 10: 1447. https://doi.org/10.3390/nu10101447