Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Fluorescence Microscopy
2.3. siRNA Knockdown
2.4. Western Blot Analysis
2.5. Cell Proliferation
2.6. Statistical Analysis
3. Results
3.1. KRAS-Mutant Cells Exhibit Higher Levels of Macropinocytosis Than Kras Wild-Type Cells
3.2. TFEB Promotes Lysosomal Degradation of Extracellular Protein without Affecting Macropinocytotic Uptake
3.3. TFEB Contributes to Macropinocytosis-Mediated Recovery of mTORC1 Activity and Cell Proliferation in Leucine-Deprived KRAS-Mutant Cells
4. Discussion
Author Contributions
Funding
Conflicts of Interest
References
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Jeong, S.; Byun, J.-K.; Cho, S.J.; Chin, J.; Lee, I.-K.; Choi, Y.-K.; Park, K.-G. Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells. Nutrients 2018, 10, 1638. https://doi.org/10.3390/nu10111638
Jeong S, Byun J-K, Cho SJ, Chin J, Lee I-K, Choi Y-K, Park K-G. Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells. Nutrients. 2018; 10(11):1638. https://doi.org/10.3390/nu10111638
Chicago/Turabian StyleJeong, Seokmin, Jun-Kyu Byun, Sung Jin Cho, Jungwook Chin, In-Kyu Lee, Yeon-Kyung Choi, and Keun-Gyu Park. 2018. "Transcription Factor Eb Is Required for Macropinocytosis-Mediated Growth Recovery of Nutrient-Deprived Kras-Mutant Cells" Nutrients 10, no. 11: 1638. https://doi.org/10.3390/nu10111638