A Low Iron Diet Protects from Steatohepatitis in a Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Experimentation
2.2. Study Approval
2.3. Cell Culture
2.4. Commercial Assays
2.5. MIP OES and LC-MS/MS
2.6. RNA Sequencing
2.7. Reverse Transcription and Real-time PCR Quantification
2.8. Seahorse Mito Stress Test
2.9. Statistics
3. Results
3.1. Metabolic Phenotyping of Mice on Diet for 3–4 Months
3.2. Iron Indices Are Not Altered by the FF Diet, and Hepatic Fat Content Is Not Affected by Dietary Iron
3.3. Iron Restriction Limits Progression of NAFLD to NASH
3.4. Iron Transcriptionally Regulates the TGF-Β Signaling Pathway
3.5. Hepatocyte–Stellate Cell Interaction is Necessary for Iron- and Fat-Induced Fibrogenesis
3.6. Oxidative Stress and Other Previously Identified Mediators Are Not Predominant Early Drivers of NASH in This Model
3.7. Iron Does Not Affect Mitochondrial Function Prior to the Initiation of Fibrosis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Salaye, L.; Bychkova, I.; Sink, S.; Kovalic, A.J.; Bharadwaj, M.S.; Lorenzo, F.; Jain, S.; Harrison, A.V.; Davis, A.T.; Turnbull, K.; et al. A Low Iron Diet Protects from Steatohepatitis in a Mouse Model. Nutrients 2019, 11, 2172. https://doi.org/10.3390/nu11092172
Salaye L, Bychkova I, Sink S, Kovalic AJ, Bharadwaj MS, Lorenzo F, Jain S, Harrison AV, Davis AT, Turnbull K, et al. A Low Iron Diet Protects from Steatohepatitis in a Mouse Model. Nutrients. 2019; 11(9):2172. https://doi.org/10.3390/nu11092172
Chicago/Turabian StyleSalaye, Lipika, Ielizaveta Bychkova, Sandy Sink, Alexander J. Kovalic, Manish S. Bharadwaj, Felipe Lorenzo, Shalini Jain, Alexandria V. Harrison, Ashley T. Davis, Katherine Turnbull, and et al. 2019. "A Low Iron Diet Protects from Steatohepatitis in a Mouse Model" Nutrients 11, no. 9: 2172. https://doi.org/10.3390/nu11092172