Novel Therapeutic Approach for the Management of Mood Disorders: In Vivo and In Vitro Effect of a Combination of L-Theanine, Melissa officinalis L. and Magnolia officinalis Rehder & E.H. Wilson
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Chemical and Drug Administration
2.3. Locomotor Activity
2.3.1. Rotarod Test
2.3.2. Hole-Board Test
2.3.3. Hot Plate Test
2.4. Anxiolytic-Like Activity
2.4.1. Light-Dark Box
2.4.2. Marbles Test
2.4.3. Novelty Suppressed Feeding Test
2.5. Antidepressant-Like Activity
Tail Suspension Test
2.6. Cell Culture
2.7. Cell Treatments
2.8. Cell Viability
2.9. Non-Competitive Sandwich ELISA Protocol for BDNF
2.10. Data and Statistical Analysis
3. Results
3.1. Anxiolytic Effect of TMM
3.2. Antidepressant-Like Activity in a Depressant-Like Paradigm
3.3. Lack of Impairment of Locomotor Behaviour and Hypersensibilization to Thermal Stimulus
3.4. Neuroprotective Effect of TMM on SH-SY5Y Neuronal Cells
3.5. Involvement of Endocannabinoid System in the Final Effect of TMM
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Borgonetti, V.; Governa, P.; Biagi, M.; Galeotti, N. Novel Therapeutic Approach for the Management of Mood Disorders: In Vivo and In Vitro Effect of a Combination of L-Theanine, Melissa officinalis L. and Magnolia officinalis Rehder & E.H. Wilson. Nutrients 2020, 12, 1803. https://doi.org/10.3390/nu12061803
Borgonetti V, Governa P, Biagi M, Galeotti N. Novel Therapeutic Approach for the Management of Mood Disorders: In Vivo and In Vitro Effect of a Combination of L-Theanine, Melissa officinalis L. and Magnolia officinalis Rehder & E.H. Wilson. Nutrients. 2020; 12(6):1803. https://doi.org/10.3390/nu12061803
Chicago/Turabian StyleBorgonetti, Vittoria, Paolo Governa, Marco Biagi, and Nicoletta Galeotti. 2020. "Novel Therapeutic Approach for the Management of Mood Disorders: In Vivo and In Vitro Effect of a Combination of L-Theanine, Melissa officinalis L. and Magnolia officinalis Rehder & E.H. Wilson" Nutrients 12, no. 6: 1803. https://doi.org/10.3390/nu12061803