Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Line and Reagent
2.2. Quantitative Reverse Transcriptase-Polymerase Chain Reaction
2.3. Investigation of Hair Growth in Experimental Animals
2.4. Immunohistochemistry
2.5. Evaluation of Mitochondrial Characteristics
2.6. mRNA Microarray Assay
2.7. Exosome Isolation
2.8. Immunocytochemistry
2.9. Statistical Analysis
3. Results
3.1. Fisetin Augments the Expression of TERT in Keratinocytes
3.2. Fisetin Promotes Hair Growth in Mice
3.3. Fisetin Activates HFSCs In Vivo
3.4. Fisetin Activates HaCaT Cells
3.5. Effects of Exosomes Derived from Fisetin-Treated HaCaT Cells on HFSCs
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Annotation Cluster | Term | Count | p-Value |
---|---|---|---|
1 | Oxidoreductase | 50 | 4.90 × 10−6 |
Oxidation-reduction process | 49 | 9.90 × 10−5 | |
2 | Secreted | 122 | 2.20 × 10−5 |
Signal | 214 | 1.60 × 10−3 | |
3 | Palmoplantar keratoderma | 10 | 5.20 × 10−6 |
Keratin | 10 | 2.10 × 10−1 | |
4 | Regulation of cell growth | 11 | 3.60 × 10−3 |
Insulin-like growth factor-binding | 4 | 4.60 × 10−2 | |
Negative regulation of cell death | 4 | 4.70 × 10−1 |
GO Term | Genes |
---|---|
Hair follicle regulation system gene | INHBA, RUNX1, TGFB2, FST, KRT17 |
Hair cycle control gene | KRT14 |
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Ogawa, M.; Udono, M.; Teruya, K.; Uehara, N.; Katakura, Y. Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion. Nutrients 2021, 13, 2087. https://doi.org/10.3390/nu13062087
Ogawa M, Udono M, Teruya K, Uehara N, Katakura Y. Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion. Nutrients. 2021; 13(6):2087. https://doi.org/10.3390/nu13062087
Chicago/Turabian StyleOgawa, Mizuki, Miyako Udono, Kiichiro Teruya, Norihisa Uehara, and Yoshinori Katakura. 2021. "Exosomes Derived from Fisetin-Treated Keratinocytes Mediate Hair Growth Promotion" Nutrients 13, no. 6: 2087. https://doi.org/10.3390/nu13062087