β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Study Design
2.2. Grip Strength Test
2.3. Treadmill Analysis
2.4. Histological Analysis of Muscle Tissue
2.5. In Vitro Study Design
2.6. Cell Viability
2.7. Western Blot Analysis
2.8. Total RNA Isolation and qPCR
2.9. Statistical Analysis
3. Results
3.1. β-Sitosterol Protects Mice from Dexamethasone-Induced Muscle Atrophy
3.2. In Vitro Verification of Muscle Loss Inhibitory Efficacy of β-Sitosterol in Dexamethasone-Induced Muscle Atrophy in C2C12 Myotube
3.3. β-Sitosterol Inhibited FoxO1-Mediated Protein Degradation, Reducing Dexamethasone-Induced Atrophy
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Hah, Y.-S.; Lee, W.K.; Lee, S.; Kim, E.J.; Lee, J.H.; Lee, S.-J.; Ji, Y.H.; Kim, S.G.; Lee, H.-H.; Hong, S.Y.; et al. β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model. Nutrients 2022, 14, 2894. https://doi.org/10.3390/nu14142894
Hah Y-S, Lee WK, Lee S, Kim EJ, Lee JH, Lee S-J, Ji YH, Kim SG, Lee H-H, Hong SY, et al. β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model. Nutrients. 2022; 14(14):2894. https://doi.org/10.3390/nu14142894
Chicago/Turabian StyleHah, Young-Sool, Won Keong Lee, Sangyeob Lee, Eun Ji Kim, Jung Hyeon Lee, Seung-Jun Lee, Yeong Ho Ji, Sang Gon Kim, Hyeong-Hwan Lee, Seo Yeon Hong, and et al. 2022. "β-Sitosterol Attenuates Dexamethasone-Induced Muscle Atrophy via Regulating FoxO1-Dependent Signaling in C2C12 Cell and Mice Model" Nutrients 14, no. 14: 2894. https://doi.org/10.3390/nu14142894