Rosmarinic Acid Improves Cognitive Abilities and Glucose Metabolism in Aged C57Bl/6N Mice While Disrupting Lipid Profile in Young Adults in a Sex-Dependent Fashion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Subjects
2.2. Treatment Administration
2.3. Experimental Procedures
2.4. Emotional Profile and Cognitive Abilities
2.4.1. Elevated Plus Maze (EPM)
2.4.2. Morris Water Maze (MWM)
2.5. Glucose Homeostasis
2.5.1. Glucose Tolerance Test (GTT)
2.5.2. Insulin Sensitivity Test (IST)
2.6. Biochemical Analysis on Trunk Blood for the Evaluation of the Glucose and Fat Metabolism
2.7. Biochemical Analysis on the Hippocampus for the Evaluation of BDNF Levels
2.8. Statistics
3. Results
3.1. Emotional Profile and Cognitive Abilities
3.1.1. Elevated Plus Maze (EPM)
3.1.2. Morris Water Maze (MWM)
3.2. Hippocampal BDNF Levels
3.3. Metabolic Characterisation
3.3.1. Body Weight
3.3.2. Glucose Tolerance Test (GTT)
3.3.3. Insulin Sensitivity Test (IST)
3.3.4. Serum Parameters
3.3.5. Plasma Parameters
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Musillo, C.; Giona, L.; Ristow, M.; Zarse, K.; Siems, K.; Di Francesco, A.; Collacchi, B.; Raggi, C.; Cirulli, F.; Berry, A. Rosmarinic Acid Improves Cognitive Abilities and Glucose Metabolism in Aged C57Bl/6N Mice While Disrupting Lipid Profile in Young Adults in a Sex-Dependent Fashion. Nutrients 2023, 15, 3366. https://doi.org/10.3390/nu15153366
Musillo C, Giona L, Ristow M, Zarse K, Siems K, Di Francesco A, Collacchi B, Raggi C, Cirulli F, Berry A. Rosmarinic Acid Improves Cognitive Abilities and Glucose Metabolism in Aged C57Bl/6N Mice While Disrupting Lipid Profile in Young Adults in a Sex-Dependent Fashion. Nutrients. 2023; 15(15):3366. https://doi.org/10.3390/nu15153366
Chicago/Turabian StyleMusillo, Chiara, Letizia Giona, Michael Ristow, Kim Zarse, Karsten Siems, Alessia Di Francesco, Barbara Collacchi, Carla Raggi, Francesca Cirulli, and Alessandra Berry. 2023. "Rosmarinic Acid Improves Cognitive Abilities and Glucose Metabolism in Aged C57Bl/6N Mice While Disrupting Lipid Profile in Young Adults in a Sex-Dependent Fashion" Nutrients 15, no. 15: 3366. https://doi.org/10.3390/nu15153366