Potential Human Health Benefits of Phaseolus vulgaris L. var Venanzio: Effects on Cancer Cell Growth and Inflammation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of P. vulgaris var. Venanzio (FV) Extracts
2.2. Analysis of Polyphenolic
2.3. Cell Culture
2.4. MTT Assay
2.5. Western Blotting Analysis
2.6. Clonogenic Assay
2.7. Trypan Blue Assay
2.8. Immunofluorescence Assay
2.9. Annexin V-FITC Staining
2.10. Senescence Assay
2.11. Determination of Reactive Oxygen Species (ROS)
2.12. Determination of PGE2 by ELISA
2.13. RT-PCR
2.14. Statistical Analysis
3. Results
3.1. Activity of P. vulgaris Extracts on Colon Cancer Cells
3.2. Effects of P. vulgaris Extracts on Inflammation and Oxidative Stress Promoted by Interleukin 1β
3.3. P. vulgaris Extracts Inhibit IL1β Effects in Colon Cancer Cells Amplified by PGE2
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Components | CW | BF |
---|---|---|
Total polyphenols | 0.84 ± 0.01 | n.d. |
Total hydroxycinnamic derivatives | 0.24 ± 0.01 | 0.11 ± 0.01 |
Gallic acid | 0.41 ± 0.02 | n.d. |
Chlorogenic acid | 0.03 ± 0.01 | n.d. |
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Bernardi, C.; Cappellucci, G.; Baini, G.; Aloisi, A.M.; Finetti, F.; Trabalzini, L. Potential Human Health Benefits of Phaseolus vulgaris L. var Venanzio: Effects on Cancer Cell Growth and Inflammation. Nutrients 2024, 16, 2534. https://doi.org/10.3390/nu16152534
Bernardi C, Cappellucci G, Baini G, Aloisi AM, Finetti F, Trabalzini L. Potential Human Health Benefits of Phaseolus vulgaris L. var Venanzio: Effects on Cancer Cell Growth and Inflammation. Nutrients. 2024; 16(15):2534. https://doi.org/10.3390/nu16152534
Chicago/Turabian StyleBernardi, Clizia, Giorgio Cappellucci, Giulia Baini, Anna Maria Aloisi, Federica Finetti, and Lorenza Trabalzini. 2024. "Potential Human Health Benefits of Phaseolus vulgaris L. var Venanzio: Effects on Cancer Cell Growth and Inflammation" Nutrients 16, no. 15: 2534. https://doi.org/10.3390/nu16152534