Impact of Lactocaseibacillus (Lactobacillus) paracasei sup. paracasei TISTR 2593 Probiotic Supplementation on the Gut Microbiome of Hypercholesterolemia Patients: A Randomized Controlled Trial
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subjects
2.2. Study Design and Treatment
2.3. Feces Collection
2.4. DNA Extraction and Sequencing
2.4.1. DNA Extraction
2.4.2. Library Preparation and Sequencing
2.5. Microbiome Analysis
2.6. Diversity Analysis of the Fecal Microbiome
2.7. Statistical Comparison Analysis
3. Results
Microbiome Profile
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Blood Parameter | Placebo | Probiotics | p-Value |
---|---|---|---|
TC (mg/dL) | 246.70 ± 40.42 | 227.75 ± 33.45 | 0.876 |
TG (mg/dL) | 157.14 ± 66.99 | 151.21 ± 41.42 | 0.115 |
HDL-C (mg/dL) | 57.00 ± 10.90 | 55.85 ± 10.83 | 0.870 |
LDL-C (mg/dL) | 163.00 ± 39.94 | 137.63 ± 29.24 | 0.027 |
Fasting plasma glucose (mg/dL) | 101.50 ± 22.61 | 96.70 ± 8.40 | 0.063 |
TC: HDL-C | 3.10 ± 1.57 | 2.48 ± 1.15 | 0.062 |
LDL-C: HDL-C | 2.68 ± 1.30 | 2.44 ± 0.59 | 0.652 |
Atherosclerosis index | 250.47 ± 40.52 | 225.38 ± 33.23 | 0.262 |
Blood Parameter | Placebo | Probiotics | ||||||
---|---|---|---|---|---|---|---|---|
Mean Difference | 95% CI | p-Value | Mean Difference | 95% CI | p-Value | |||
TC (mg/dL) | 15.22 | −3.20 | 42.50 | 0.304 | −5.75 | −18.06 | 22.64 | 0.565 |
TG (mg/dL) | 11.06 | −10.73 | 30.43 | 0.563 | 7.71 | −21.53 | 22.71 | 0.496 |
HDL-C (mg/dL) | 4.52 | −3.52 | 13.42 | 0.357 | 2.50 | −2.37 | 12.49 | 0.342 |
LDL-C (mg/dL) | 11.39 | −5.29 | 35.09 | 0.532 | −17.52 | −30.32 | 2.08 | 0.038 |
Fasting plasma glucose (mg/dL) | 5.41 | 2.27 | 10.83 | 0.014 | 0.88 | −2.84 | 4.60 | 0.492 |
TC: HDL-C | 0.20 | −1.02 | 1.22 | 0.785 | −0.46 | −1.59 | 0.11 | 0.712 |
LDL-C: HDL-C | −0.28 | −1.44 | 0.84 | 0.446 | −0.53 | −1.78 | 1.32 | 0.361 |
Atherosclerosis index | 19.35 | 2.26 | 46.21 | 0.293 | −7.77 | −24.68 | 18.87 | 0.484 |
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Nuankham, K.; Sitdhipol, J.; Chonpathompikunlert, P.; Khongrum, J.; Kittichaiworakul, R.; Noisagul, P.; Thongkumkoon, P.; Kampoun, T.; Dissook, S. Impact of Lactocaseibacillus (Lactobacillus) paracasei sup. paracasei TISTR 2593 Probiotic Supplementation on the Gut Microbiome of Hypercholesterolemia Patients: A Randomized Controlled Trial. Nutrients 2024, 16, 2916. https://doi.org/10.3390/nu16172916
Nuankham K, Sitdhipol J, Chonpathompikunlert P, Khongrum J, Kittichaiworakul R, Noisagul P, Thongkumkoon P, Kampoun T, Dissook S. Impact of Lactocaseibacillus (Lactobacillus) paracasei sup. paracasei TISTR 2593 Probiotic Supplementation on the Gut Microbiome of Hypercholesterolemia Patients: A Randomized Controlled Trial. Nutrients. 2024; 16(17):2916. https://doi.org/10.3390/nu16172916
Chicago/Turabian StyleNuankham, Kamonsri, Jaruwan Sitdhipol, Pennapa Chonpathompikunlert, Jurairat Khongrum, Romteera Kittichaiworakul, Pitiporn Noisagul, Patcharawadee Thongkumkoon, Tanyaluck Kampoun, and Sivamoke Dissook. 2024. "Impact of Lactocaseibacillus (Lactobacillus) paracasei sup. paracasei TISTR 2593 Probiotic Supplementation on the Gut Microbiome of Hypercholesterolemia Patients: A Randomized Controlled Trial" Nutrients 16, no. 17: 2916. https://doi.org/10.3390/nu16172916