Revealing Interactions of Gut Microbiota and Metabolite in Confined Environments Using High-Throughput Sequencing and Metabolomic Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Subject
2.2. Confined Environment Simulation
2.3. Sample Collection
2.4. High-Throughput Sequencing and Bioinformatics Analysis
2.5. Untargeted Metabolomics Analysis of Urine
3. Results
3.1. Enterotypes Analysis of Intestinal Microbiota in Subjects
3.2. Effects of Confined Environment on Intestinal Microbiota of Different Enterotypes
3.3. Differences in the Response of Different Intestinal Microbiota to Confined Environment
3.4. Untargeted Metabolomics Analysis
3.5. Differential Metabolite Analysis
3.6. Effect of Confined Environment on the Relative Abundance of Uric Acid
3.7. Correlation Analysis of Intestinal Microbiota with Metabolites
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, Z.; Xu, H.; Song, X.; Chen, Z.; Wang, G.; Yang, Y.; Zhu, B.; Ai, L.; Liu, C.; Zhang, Y.; et al. Revealing Interactions of Gut Microbiota and Metabolite in Confined Environments Using High-Throughput Sequencing and Metabolomic Analysis. Nutrients 2024, 16, 2998. https://doi.org/10.3390/nu16172998
Wang Z, Xu H, Song X, Chen Z, Wang G, Yang Y, Zhu B, Ai L, Liu C, Zhang Y, et al. Revealing Interactions of Gut Microbiota and Metabolite in Confined Environments Using High-Throughput Sequencing and Metabolomic Analysis. Nutrients. 2024; 16(17):2998. https://doi.org/10.3390/nu16172998
Chicago/Turabian StyleWang, Ziying, Haodan Xu, Xin Song, Zheng Chen, Guangqiang Wang, Yijin Yang, Beiwei Zhu, Lianzhong Ai, Chenxi Liu, Yaxuan Zhang, and et al. 2024. "Revealing Interactions of Gut Microbiota and Metabolite in Confined Environments Using High-Throughput Sequencing and Metabolomic Analysis" Nutrients 16, no. 17: 2998. https://doi.org/10.3390/nu16172998