Anti-Inflammatory Effect of Dietary Pentadecanoic Fatty Acid Supplementation on Inflammatory Bowel Disease in SAMP1/YitFc Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Measures to Improve Reproducibility
2.2. Spontaneous Ileitis and DSS Colitis in SAMP Mice
2.3. Diet
2.4. Experimental Design
2.5. DSS Induced Colitis and Colitis Severity Assessment
2.6. Analysis of Intestinal Inflammation and Gut Permeability In Vivo
2.7. Histopathological Analysis
2.8. nCounter Nanostring Gene Expression Analysis
2.9. Data Analysis
3. Results
3.1. Three-Week C:15 Supplementation Prevented the Occurrence of Severe DSS-Colitis in SAMP Mice
3.2. Six-Week C:15 Supplementation Reduced the Endoscopic Severity of DSS-Colitis in SAMP Mice
3.3. Six-Week Supplementation Reduces the Severity of Advanced Ileitis in SAMP Mice
3.4. Three-Week C:15 Supplementation Altered the Expression of Various Genes Involved in Different Immunological Pathways in SAMP Mice
4. Discussion
5. Conclusions and Future Directions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Experiment | DSS Induced Colitis | DSS Induced Colitis | Chronic Ileitis |
---|---|---|---|
C:15 administration | Short term (3 weeks) | Long term (6 weeks) | 6 weeks |
Mouse (age) | SAMP/YitFc (14 weeks) | SAMP/YitFc (14 weeks) | SAMP/YitFc (24 weeks) |
Comparison | C:15(1X) vs. Control | C:15(1X) vs. (2X) vs. Control | C:15(1X) vs. Control |
% Residual Body weight * | C:15: 103.1 ± 2.0 Control: 92.0 ± 2.8 | C:15(1X): 97.5 ± 2.9 C:15(2X): 99.5 ± 2.5 Control: 97.1 ± 1.7 | C:15: 105.2 ± 1.4 Control: 96.3 ± 1.3 |
MPO U/mg Log2 * | C:15: 2.5 ± 0.5 Control: 5.2 ± 0.6 | C:15(1X): 3.1 ± 0.2 C:15(2X): 3.2 ± 0.9 Control: 7.1 ± 0.1 | C:15: 4.2 ± 0.5 Control: 6.2 ± 0.9 |
Fluorescence Log2 * | C:15 18.6 ± 0.7 Control: 21.4 ± 0.6 | C:15(1X): 19.0 ± 2.1 C:15(2X): 17.6 ± 0.5 Control: 24.0 ± 0.5 | C:15: 15.4 ± 0.2 Control: 15.8 ± 0.2 |
Colon length * | C:15: 7.0 ± 0.6 Control: 5.6 ± 0.4 | C:15(1X): 6.5 ± 0.4 C:15(2X): 6.3 ± 0.5 Control: 5.6 ± 0.3 | NA |
Colonoscopy * | C:15: 3.1 ± 1.0 Control: 4.4 ± 1.5 | C:15(1X): 1.6 ± 0.8 C:15(2X): 1.6 ± 0.8 Control: 4.7 ± 0.9 | NA |
Colon Histology * | C:15: 11.0 ± 4.3 Control: 17.8 ± 1.6 | C:15(1X): 10.9 ± 4.8 C:15(2X): 6.3 ± 2.3 Control: 8.9 ± 3.4 | NA |
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Singh, D.; Mehghini, P.; Rodriguez-Palacios, A.; Di Martino, L.; Cominelli, F.; Basson, A.R. Anti-Inflammatory Effect of Dietary Pentadecanoic Fatty Acid Supplementation on Inflammatory Bowel Disease in SAMP1/YitFc Mice. Nutrients 2024, 16, 3031. https://doi.org/10.3390/nu16173031
Singh D, Mehghini P, Rodriguez-Palacios A, Di Martino L, Cominelli F, Basson AR. Anti-Inflammatory Effect of Dietary Pentadecanoic Fatty Acid Supplementation on Inflammatory Bowel Disease in SAMP1/YitFc Mice. Nutrients. 2024; 16(17):3031. https://doi.org/10.3390/nu16173031
Chicago/Turabian StyleSingh, Drishtant, Paola Mehghini, Alexander Rodriguez-Palacios, Luca Di Martino, Fabio Cominelli, and Abigail Raffner Basson. 2024. "Anti-Inflammatory Effect of Dietary Pentadecanoic Fatty Acid Supplementation on Inflammatory Bowel Disease in SAMP1/YitFc Mice" Nutrients 16, no. 17: 3031. https://doi.org/10.3390/nu16173031