The Addition of Liquid Fructose to a Western-Type Diet in LDL-R−/− Mice Induces Liver Inflammation and Fibrogenesis Markers without Disrupting Insulin Receptor Signalling after an Insulin Challenge
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Diets
2.2. Blood and Plasma Analysis
2.3. Histological Studies
2.4. RNA Preparation and Analysis
2.5. Western Blot Analysis
2.6. Statistical Analysis
3. Results
3.1. Western Diet ± Liquid Fructose Supplementation Does Not Trigger Visceral White Adipose Tissue Inflammation in LDL-R−/− Mice
3.2. A Western-Type Diet Induces Hepatic Inflammation and Liquid Fructose Supplementation Enhances This Effect
3.3. Addition of a Liquid Fructose Supplement to a Western-Type Diet Increases Fibrogenesis Markers in the Liver of LDL-R−/− Mice
3.4. Fructose Supplementation and a Western Diet Reduce Basal Insulin Receptor Substrate-2 (IRS-2) Expression but Do Not Impair Insulin-Stimulated Akt Phosphorylation in the Liver of LDL-R−/− Mice
4. Discussion
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sangüesa, G.; Baena, M.; Hutter, N.; Montañés, J.C.; Sánchez, R.M.; Roglans, N.; Laguna, J.C.; Alegret, M. The Addition of Liquid Fructose to a Western-Type Diet in LDL-R−/− Mice Induces Liver Inflammation and Fibrogenesis Markers without Disrupting Insulin Receptor Signalling after an Insulin Challenge. Nutrients 2017, 9, 278. https://doi.org/10.3390/nu9030278
Sangüesa G, Baena M, Hutter N, Montañés JC, Sánchez RM, Roglans N, Laguna JC, Alegret M. The Addition of Liquid Fructose to a Western-Type Diet in LDL-R−/− Mice Induces Liver Inflammation and Fibrogenesis Markers without Disrupting Insulin Receptor Signalling after an Insulin Challenge. Nutrients. 2017; 9(3):278. https://doi.org/10.3390/nu9030278
Chicago/Turabian StyleSangüesa, Gemma, Miguel Baena, Natalia Hutter, José Carlos Montañés, Rosa María Sánchez, Núria Roglans, Juan Carlos Laguna, and Marta Alegret. 2017. "The Addition of Liquid Fructose to a Western-Type Diet in LDL-R−/− Mice Induces Liver Inflammation and Fibrogenesis Markers without Disrupting Insulin Receptor Signalling after an Insulin Challenge" Nutrients 9, no. 3: 278. https://doi.org/10.3390/nu9030278