Lifetime Exposure to a Constant Environment Amplifies the Impact of a Fructose-Rich Diet on Glucose Homeostasis during Pregnancy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Diets
2.2. Regular Monitoring of Body Weight and Plasma Metabolites
2.3. Oral Glucose Tolerance Tests (OGTT)
2.4. Determination of Body Composition and Tissue Collection
2.5. RNA Extraction and Determination of Hepatic Gene Expression
2.6. Statistical Analysis
3. Results and Discussion
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Age/Pregnancy Stage | Gen0-C | Gen0-F | Gen1-C | Gen1-F |
---|---|---|---|---|
Body Weight (g) | ||||
Week 8/Pre-diet | 200.6 ± 3.3 | 204.1 ± 3.1 | 225.1 ± 6.3 † | 226.4 ± 5.7 † |
Week 9 | 227.0 ± 2.0 | 238.3 ± 2.9 | 254.5 ± 6.6 † | 252.3 ± 6.5 † |
Week 10 | 247.3 ± 2.3 | 257.2 ± 3.1 | 280.7 ± 6.8 † | 285.9 ± 7.7 † |
Week 11/Pre-mating | 261.5 ± 3.0 | 277.7 ± 4.7 | 305.5 ± 7.4 † | 316.3 ± 9.3 † |
Week 12/ Early pregnancy | 290.5 ± 2.3 | 304.9 ± 5.3 | 332.2 ± 6.8 † | 352.3 ± 12.9 † |
Week 13/ Mid pregnancy | 329.4 ± 5.1 | 337.6 ± 5.9 | 383.8 ± 7.6 † | 393.3 ± 14.3 † |
Week 14/ Late pregnancy | 386.5 ± 5.4 | 403.1 ± 6.7 | 428.7 ± 12.3 † | 444.4 ± 15.6 † |
Body Composition (%) | ||||
Fat mass at GD21 | 11.2 ± 0.7 | 15.2 ± 1.0 * | 11.4 ± 0.5 | 16.7 ± 1.3 * |
Feto-placental unit | ||||
Number of pups | 15.5 ± 1.7 | 16.0 ± 3.2 | 16.3 ± 2.3 | 17.0 ± 3.3 |
Placental weight (g) | 0.53 ± 0.02 | 0.48 ± 0.02 | 0.57 ± 0.02 | 0.47 ± 0.01 * |
Fetal weight (g) | 3.88 ± 0.19 | 3.36 ± 0.14 * | 4.07 ± 0.05 | 3.57 ± 0.09 * |
The ratio of placental:fetal weight | 0.14 ± 0.01 | 0.15 ± 0.01 | 0.14 ± 0.00 | 0.13 ± 0.01 |
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Song, A.; Astbury, S.; Hoedl, A.; Nielsen, B.; Symonds, M.E.; Bell, R.C. Lifetime Exposure to a Constant Environment Amplifies the Impact of a Fructose-Rich Diet on Glucose Homeostasis during Pregnancy. Nutrients 2017, 9, 327. https://doi.org/10.3390/nu9040327
Song A, Astbury S, Hoedl A, Nielsen B, Symonds ME, Bell RC. Lifetime Exposure to a Constant Environment Amplifies the Impact of a Fructose-Rich Diet on Glucose Homeostasis during Pregnancy. Nutrients. 2017; 9(4):327. https://doi.org/10.3390/nu9040327
Chicago/Turabian StyleSong, Aleida, Stuart Astbury, Abha Hoedl, Brent Nielsen, Michael E. Symonds, and Rhonda C. Bell. 2017. "Lifetime Exposure to a Constant Environment Amplifies the Impact of a Fructose-Rich Diet on Glucose Homeostasis during Pregnancy" Nutrients 9, no. 4: 327. https://doi.org/10.3390/nu9040327