Next Article in Journal
Occurrence and Ear Damage of Helicoverpa zea on Transgenic Bacillus thuringiensis Maize in the Field in Texas, U.S. and Its Susceptibility to Vip3A Protein
Previous Article in Journal
Co-Occurrence of Beauvericin and Enniatins in Edible Vegetable Oil Samples, China
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Toxins
Volume 11
Issue 2
10.3390/toxins11020101
toxins-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry

by
Jenna Tonacini
1,
Dario Stephan
1,
Guido Vogel
2,
Marc-André Avondet
3,
Franka Kalman
1,
Julien Crovadore
4,
François Lefort
4 and
Bruno Schnyder
1,*
1
Institute of Life Technologies, University of Applied Sciences, HES-SO Valais/Wallis, Sion 1950, Switzerland
2
Mabritec AG, Food Technology, Riehen 4125, Switzerland
3
Spiez Laboratory, Federal Office for Civil Protection, Spiez 3700, Switzerland
4
Plants and Pathogens Group, Research Institute Land Nature Environment School of, Architecture and Landscape HEPIA HES-SO University of Applied Sciences and Arts Western Switzerland, 150 route de Presinge, 1254 Geneva/Jussy, Switzerland
*
Author to whom correspondence should be addressed.
Toxins 2019, 11(2), 101; https://doi.org/10.3390/toxins11020101
Submission received: 11 December 2018 / Revised: 1 February 2019 / Accepted: 6 February 2019 / Published: 9 February 2019
Browse Figures
Versions Notes

Abstract

Routine identification of pathogens by MALDI-TOF MS (matrix-assisted laser desorption ionisation time-of-flight mass spectrometry) is based on the fingerprint of intracellular proteins. This work evaluated the use of MALDI-TOF MS for the identification of extracellular pathogen factors. A Staphylococcus aureus isolate from a food contaminant was exponentially grown in liquid cultures. Secreted proteins were collected using methanol– chloroform precipitation and analysed by MALDI-TOF MS. A main peak m/z 28,250 was demonstrated, which was identified as S.aureus enterotoxin type B (SEB) by using the pure authentic SEB reference of 28.2 kDa and by amino acid sequence analysis. SEB was also detected in this intact form following pasteurization and cooking treatments. Further application of the elaborated MALDI-TOF MS protocol resulted in the detection of SEA at m/z 27,032 and SEC at m/z 27,629. In conclusion, a simple sample preparation from S.aureus cultures and an easy-to-perform identification of pathogen factors SE in intact form represents a promising next-generation application of MALDI-TOF MS.
Keywords: staphylococcal enterotoxins; superantigen; MALDI-TOF MS staphylococcal enterotoxins; superantigen; MALDI-TOF MS

Share and Cite

MDPI and ACS Style

Tonacini, J.; Stephan, D.; Vogel, G.; Avondet, M.-A.; Kalman, F.; Crovadore, J.; Lefort, F.; Schnyder, B. Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry. Toxins 2019, 11, 101. https://doi.org/10.3390/toxins11020101

AMA Style

Tonacini J, Stephan D, Vogel G, Avondet M-A, Kalman F, Crovadore J, Lefort F, Schnyder B. Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry. Toxins. 2019; 11(2):101. https://doi.org/10.3390/toxins11020101

Chicago/Turabian Style

Tonacini, Jenna, Dario Stephan, Guido Vogel, Marc-André Avondet, Franka Kalman, Julien Crovadore, François Lefort, and Bruno Schnyder. 2019. "Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry" Toxins 11, no. 2: 101. https://doi.org/10.3390/toxins11020101

APA Style

Tonacini, J., Stephan, D., Vogel, G., Avondet, M.-A., Kalman, F., Crovadore, J., Lefort, F., & Schnyder, B. (2019). Intact Staphylococcus Enterotoxin SEB from Culture Supernatant Detected by MALDI-TOF Mass Spectrometry. Toxins, 11(2), 101. https://doi.org/10.3390/toxins11020101

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop