Unliganded Progesterone Receptor Governs Estrogen Receptor Gene Expression by Regulating DNA Methylation in Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Unliganded PR Is Required to Maintain ESR1 Gene Basal Expression in Breast Cancer Cells
2.2. PR Binds to the ESR1 Locus in Hormone-Free Breast Cancer Cells
2.3. Rescue of PR Does Not Restore ESR1 Gene Expression in PR-Deficient Cells
2.4. Lack of PR Affects DNA Methylation at the ESR1 Promoter
2.5. DNA Methylation Impedes PR Binding to Hormone Responsive Elements
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Chemical Treatments
4.2. Lentivirus Preparation and Infection
4.3. Reverse Transcription and Quantitative PCR
4.4. Western Blotting
4.5. Chromatin Immunoprecipitation
4.6. DNA Methylation
4.7. Electrophoresis Mobility-Shift Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Verde, G.; De Llobet, L.I.; Wright, R.H.G.; Quilez, J.; Peiró, S.; Le Dily, F.; Beato, M. Unliganded Progesterone Receptor Governs Estrogen Receptor Gene Expression by Regulating DNA Methylation in Breast Cancer Cells. Cancers 2018, 10, 371. https://doi.org/10.3390/cancers10100371
Verde G, De Llobet LI, Wright RHG, Quilez J, Peiró S, Le Dily F, Beato M. Unliganded Progesterone Receptor Governs Estrogen Receptor Gene Expression by Regulating DNA Methylation in Breast Cancer Cells. Cancers. 2018; 10(10):371. https://doi.org/10.3390/cancers10100371
Chicago/Turabian StyleVerde, Gaetano, Lara I. De Llobet, Roni H.G. Wright, Javier Quilez, Sandra Peiró, François Le Dily, and Miguel Beato. 2018. "Unliganded Progesterone Receptor Governs Estrogen Receptor Gene Expression by Regulating DNA Methylation in Breast Cancer Cells" Cancers 10, no. 10: 371. https://doi.org/10.3390/cancers10100371