Loss of Myeloid-Specific TGF-β Signaling Decreases CTHRC1 to Downregulate bFGF and the Development of H1993-Induced Osteolytic Bone Lesions
Abstract
:1. Introduction
2. Results
2.1. Osteolytic Bone Lesions Were Inhibited in Tgfbr2LysMCre KO Mice
2.2. Myeloid-Specific TGF-β Signaling Promoted H1993-Induced Bone Lesions and bFGF Expression
2.3. Loss of TGFBR2 in Osteoclasts Decreased the Expression and Secretion of CTHRC1
2.4. CTHRC1 Induced the Expression of bFGF in Osteoblasts and Osteoblastogenesis
2.5. CTHRC1 Promoted bFGF Expression in a Wnt/β-Catenin-Dependent Manner
3. Discussion
4. Materials and Methods
4.1. Cells Cultures and Animals
4.2. Injections and Radiographic Imaging
4.3. Histology, Histomorphometry, and Immunohistochemistry
4.4. Protein Extraction and Western Blots
4.5. RNA Extraction and Quantitative Real-Time RT-PCR (qRT-PCR)
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ganguly, S.S.; Daft, P.G.; Cao, J.; Meng, X.; Zhong, Z.A.; Vander Ark, A.; Meadows, A.; Madaj, Z.; Williams, B.; Li, X. Loss of Myeloid-Specific TGF-β Signaling Decreases CTHRC1 to Downregulate bFGF and the Development of H1993-Induced Osteolytic Bone Lesions. Cancers 2018, 10, 463. https://doi.org/10.3390/cancers10120463
Ganguly SS, Daft PG, Cao J, Meng X, Zhong ZA, Vander Ark A, Meadows A, Madaj Z, Williams B, Li X. Loss of Myeloid-Specific TGF-β Signaling Decreases CTHRC1 to Downregulate bFGF and the Development of H1993-Induced Osteolytic Bone Lesions. Cancers. 2018; 10(12):463. https://doi.org/10.3390/cancers10120463
Chicago/Turabian StyleGanguly, Sourik S., Paul G. Daft, Jingchen Cao, Xiangqi Meng, Zhendong A. Zhong, Alexandra Vander Ark, Austin Meadows, Zach Madaj, Bart Williams, and Xiaohong Li. 2018. "Loss of Myeloid-Specific TGF-β Signaling Decreases CTHRC1 to Downregulate bFGF and the Development of H1993-Induced Osteolytic Bone Lesions" Cancers 10, no. 12: 463. https://doi.org/10.3390/cancers10120463