Tannic Acid Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Prostate Cancer
Abstract
:1. Introduction
2. Results
2.1. Tannic Acid Treatment Suppresses Proliferation and Clonogelnicity of Prostate Cancer Cells
2.2. Tannic Acid Treatment Upregulates the Expression of ER Stress Regulatory Proteins
2.3. TA Treatment Effectively Arrests the Cell Cycle and Modulates Cell Cycle Regulatory Proteins
2.4. Tannic Acid Treatment Induced Apoptosis via ER Stress Mediated Signaling
2.5. Tannic Acid Treatment Inhibited Migratory and Invasive Potentials of Prostate Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Materials and Reagents
4.2. Cell Proliferation Assay
4.3. Colony Formation Assay
4.4. Cell Cycle Analysis
4.5. Wound Healing Assay
4.6. Cell Migration Assay
4.7. Cell Invasion Assay
4.8. cDNA Synthesis and Quantitative mRNA Expression by Real-Time PCR
4.9. mRNA Purification, Ethanol Precipitation and Micro Array Studies
4.10. Western Blot Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Genes | Forward Primers | Reverse Primers |
---|---|---|
BiP | 5′-CATGGTTCTCACTAAAATGAAAGG-3′ | 5′-GCTGGTACAGTAACAACTG-3′ |
CHOP/GADD153 | 5′-GCCTTTCTCCTTTGGGACACTGTCCAGC-3′ | 5′-CTCGGCGAGTCGCCTCTACTTCCC-3′ |
ATF4 | 5′-GCATGCTCTGTTTCGAATGGA-3′ | 5′-CCAACGTGGTCAAGAGCTCAT-3′ |
PERK | 5′-TCTTGGTTGGGTCTGATGAAT-3′ | 5′-GATGTTCTTGCTGTAGTGGGGG-3′ |
EIF2S1 | 5′-ACGACAACCCTGGAGAGAAC-3′ | 5′-TATGACATTCCAAGGCCGACA-3′ |
E-Cadherin | 5′-TGGAGGAATTCTTGCTTTGC-3′ | 5′-CGTACATGTCAGCCAGCTTC-3′ |
MMP2 | 5′-TCTCCTGACATTGACCTTGGC-3′ | 5′-CAAGGTGCTGGCTGAGTAGATC-3′ |
MMP9 | 5′-TTGACAGCGACAAGAAGTGG-3′ | 5′-GCCATTCACGTCGTCCTTAT-3′ |
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Nagesh, P.K.B.; Hatami, E.; Chowdhury, P.; Kashyap, V.K.; Khan, S.; Hafeez, B.B.; Chauhan, S.C.; Jaggi, M.; Yallapu, M.M. Tannic Acid Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Prostate Cancer. Cancers 2018, 10, 68. https://doi.org/10.3390/cancers10030068
Nagesh PKB, Hatami E, Chowdhury P, Kashyap VK, Khan S, Hafeez BB, Chauhan SC, Jaggi M, Yallapu MM. Tannic Acid Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Prostate Cancer. Cancers. 2018; 10(3):68. https://doi.org/10.3390/cancers10030068
Chicago/Turabian StyleNagesh, Prashanth K.B., Elham Hatami, Pallabita Chowdhury, Vivek K. Kashyap, Sheema Khan, Bilal B. Hafeez, Subhash C. Chauhan, Meena Jaggi, and Murali M. Yallapu. 2018. "Tannic Acid Induces Endoplasmic Reticulum Stress-Mediated Apoptosis in Prostate Cancer" Cancers 10, no. 3: 68. https://doi.org/10.3390/cancers10030068