Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells
Abstract
:1. Introduction
2. Results
2.1. Expression of Chemokine Receptors is Heterogeneous in both ALCL and cHL
2.2. CCL17 and CCL22 are Frequently Expressed by cHL But Only Very Rarely in ALCL Tumor Cells
2.3. cHL Has a More Abundant Microenvironment and HRS Cells Are More Effective for the Induction of T-Cell Rosettes Compared with ALCL
2.4. Filopodia-like Structures Occur More Frequently and Are Longer in the HRS Cells of cHL Cell Lines Than Those in ALCL Cell Lines
2.5. ALCL Cells Actively Migrate Towards an FCS Gradient and Move Faster in Bovine Collagen Type I Gel than HRS Cells
3. Discussion
4. Materials and Methods
4.1. Immunohistochemistry
4.2. Human Chemokine Array
4.3. Analysis of Cell Motility in A Collagen Type I Gel
4.4. Transwell Migration Experiments
4.5. Co-Culture Experiments and Cluster Formation Assay
4.6. Analysis of Filopodia-Like Structures in a Collagen Type I Gel
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Goncharova, O.; Flinner, N.; Bein, J.; Döring, C.; Donnadieu, E.; Rikirsch, S.; Herling, M.; Küppers, R.; Hansmann, M.-L.; Hartmann, S. Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells. Cancers 2019, 11, 1484. https://doi.org/10.3390/cancers11101484
Goncharova O, Flinner N, Bein J, Döring C, Donnadieu E, Rikirsch S, Herling M, Küppers R, Hansmann M-L, Hartmann S. Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells. Cancers. 2019; 11(10):1484. https://doi.org/10.3390/cancers11101484
Chicago/Turabian StyleGoncharova, Olga, Nadine Flinner, Julia Bein, Claudia Döring, Emmanuel Donnadieu, Sandy Rikirsch, Marco Herling, Ralf Küppers, Martin-Leo Hansmann, and Sylvia Hartmann. 2019. "Migration Properties Distinguish Tumor Cells of Classical Hodgkin Lymphoma from Anaplastic Large Cell Lymphoma Cells" Cancers 11, no. 10: 1484. https://doi.org/10.3390/cancers11101484