The Protein Neddylation Inhibitor MLN4924 Suppresses Patient-Derived Glioblastoma Cells via Inhibition of ERK and AKT Signaling
Abstract
:1. Introduction
2. Results
2.1. The Neddylation Pathway is Overactivated in Patient-Derived Glioblastoma Specimens
2.2. MLN4924 Inhibits Proliferation and Induces Apoptosis in Glioblastoma Cell Lines
2.3. MLN4924 Suppresses Cullin 1 Neddylation in Patient-Derived Glioblastoma Stem Cells
2.4. Response of MLN4924 is Related to ERK and AKT Signaling Pathway
2.5. Sensitivity of MLN4924 is Associated with Upregulation of ERK and AKT Signaling
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Culture Conditions
4.2. Patient-Derived Glioblastoma Specimens and Primary Cell Culture
4.3. Drugs
4.4. Immunofluorescence Staining and High Content Analysis
4.5. Western Blot Assay
4.6. Immunohistochemistry
4.7. Flow Cytometry-Based Apoptosis Detection
4.8. RNA Sequencing
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Han, S.; Shin, H.; Oh, J.-W.; Oh, Y.J.; Her, N.-G.; Nam, D.-H. The Protein Neddylation Inhibitor MLN4924 Suppresses Patient-Derived Glioblastoma Cells via Inhibition of ERK and AKT Signaling. Cancers 2019, 11, 1849. https://doi.org/10.3390/cancers11121849
Han S, Shin H, Oh J-W, Oh YJ, Her N-G, Nam D-H. The Protein Neddylation Inhibitor MLN4924 Suppresses Patient-Derived Glioblastoma Cells via Inhibition of ERK and AKT Signaling. Cancers. 2019; 11(12):1849. https://doi.org/10.3390/cancers11121849
Chicago/Turabian StyleHan, Suji, Hyemi Shin, Jeong-Woo Oh, Yun Jeong Oh, Nam-Gu Her, and Do-Hyun Nam. 2019. "The Protein Neddylation Inhibitor MLN4924 Suppresses Patient-Derived Glioblastoma Cells via Inhibition of ERK and AKT Signaling" Cancers 11, no. 12: 1849. https://doi.org/10.3390/cancers11121849