NGFI-A Binding Protein 2 Promotes EGF-Dependent HNSCC Cell Invasion
Abstract
:1. Introduction
2. Results
2.1. EGF Upregulates EGR1 Expression in HNSCC Cells
2.2. NAB2 Knockdown in HNSCC Cells Alleviates EGF-Dependent Increase of Matrigel Invasion
2.3. NAB2 Knockdown Relieves the EGF-Dependent Upregulation of MMP2 and MMP9
2.4. Molecular Signatures Reflected by the TCGA Dataset of HNSCC Patient Tumor Tissues
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Cell Culture
4.3. Transfection of siRNA or Overexpression Vectors
4.4. Real-Time PCR (qRT-PCR)
4.5. Western Blot Analysis
4.6. Matrigel Invasion Assays
4.7. Chromatin Immunoprecipitation (ChIP) Analysis
4.8. Promoter-Luciferase Assays
4.9. TCGA RNAseq Data Analysis
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Kim, J.; Kang, S.-M.; Oh, S.Y.; Lee, H.-J.; Lee, I.; Hwang, J.-C.; Hong, S.-H. NGFI-A Binding Protein 2 Promotes EGF-Dependent HNSCC Cell Invasion. Cancers 2019, 11, 315. https://doi.org/10.3390/cancers11030315
Kim J, Kang S-M, Oh SY, Lee H-J, Lee I, Hwang J-C, Hong S-H. NGFI-A Binding Protein 2 Promotes EGF-Dependent HNSCC Cell Invasion. Cancers. 2019; 11(3):315. https://doi.org/10.3390/cancers11030315
Chicago/Turabian StyleKim, Jinkyung, Sung-Min Kang, Su Young Oh, Heon-Jin Lee, Inhan Lee, Jae-Chan Hwang, and Su-Hyung Hong. 2019. "NGFI-A Binding Protein 2 Promotes EGF-Dependent HNSCC Cell Invasion" Cancers 11, no. 3: 315. https://doi.org/10.3390/cancers11030315