De Novo T790M Mutation in an L858R Epidermal Growth Factor Receptor Mutant-Associated Lung Adenocarcinoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Generation of Transgenic Mice Carrying Lung-Specific TetO-[L858R]-hEGFR or TetO-[L858R+T790M]-hEGFR BAC Transgenes
2.2. Induction of hEGFR Transgene Expression
2.3. Induction of Lung Tumors in TG Mice on Doxycycline
2.4. Acquisition of De Novo T790M Mutant in [L858R]-hEGFR Bitransgenic Mouse Lung Tumor
3. Discussion
4. Materials and Methods
4.1. Preparation of [L858R] Human EGFR and [L858R+T790M] Human EGFR Recombinant Bacterial Artificial Chromosome Expression Constructs
4.2. Preparation of the CCSP-rtTA Recombinant BAC Expression Construct
4.3. Ethical Statement
4.4. Screening of Lung Tumors by Micro-Computed Tomography (CT)
4.5. Plasma Sampling
4.6. Lung Tissue Examination
4.7. Biochemical Analysis
4.8. Western Blotting
4.9. Evaluation of Gene Expression by PCR
4.10. Statistical Analysis
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Fujiwara, T.; Kobayashi, T.; Yasuma, T.; D’Alessandro-Gabazza, C.N.; Toda, M.; Fujimoto, H.; Fujiwara, K.; Takeshita, A.; Nishihama, K.; Okano, T.; et al. De Novo T790M Mutation in an L858R Epidermal Growth Factor Receptor Mutant-Associated Lung Adenocarcinoma. Cancers 2020, 12, 3074. https://doi.org/10.3390/cancers12103074
Fujiwara T, Kobayashi T, Yasuma T, D’Alessandro-Gabazza CN, Toda M, Fujimoto H, Fujiwara K, Takeshita A, Nishihama K, Okano T, et al. De Novo T790M Mutation in an L858R Epidermal Growth Factor Receptor Mutant-Associated Lung Adenocarcinoma. Cancers. 2020; 12(10):3074. https://doi.org/10.3390/cancers12103074
Chicago/Turabian StyleFujiwara, Takumi, Tetsu Kobayashi, Taro Yasuma, Corina N. D’Alessandro-Gabazza, Masaaki Toda, Hajime Fujimoto, Kentaro Fujiwara, Atsuro Takeshita, Kota Nishihama, Tomohito Okano, and et al. 2020. "De Novo T790M Mutation in an L858R Epidermal Growth Factor Receptor Mutant-Associated Lung Adenocarcinoma" Cancers 12, no. 10: 3074. https://doi.org/10.3390/cancers12103074