Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model
Abstract
:1. Introduction
2. Results
2.1. Formation and Growth of Pancreatic TSs in a Collagen Matrix
2.2. Expression of EMT-Related Factors in TSs
2.3. Differential Modes of Cancer Cell Migration and Focal Adhesion
2.4. Remodeling of the Collagen and Fibronectin Matrix
2.5. Expression of MMPs and Cleaved Collagen
2.6. Migration of Cancer Cells out of the Collagen Matrix and Contact with PSCs
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Fabrication of the Polydimethylsiloxane (PDMS) Microchannel Chip
4.3. Three-Dimensional Spheroid Culture in the Microchannel Chip
4.4. Immunofluorescence Staining and Image Acquisition
4.5. Image Analysis
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Kim, S.-K.; Jang, S.D.; Kim, H.; Chung, S.; Park, J.K.; Kuh, H.-J. Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model. Cancers 2020, 12, 1305. https://doi.org/10.3390/cancers12051305
Kim S-K, Jang SD, Kim H, Chung S, Park JK, Kuh H-J. Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model. Cancers. 2020; 12(5):1305. https://doi.org/10.3390/cancers12051305
Chicago/Turabian StyleKim, Seul-Ki, So Dam Jang, Hyunho Kim, Seok Chung, Jong Kook Park, and Hyo-Jeong Kuh. 2020. "Phenotypic Heterogeneity and Plasticity of Cancer Cell Migration in a Pancreatic Tumor Three-Dimensional Culture Model" Cancers 12, no. 5: 1305. https://doi.org/10.3390/cancers12051305