Metabolic Reprogramming in Metastatic Melanoma with Acquired Resistance to Targeted Therapies: Integrative Metabolomic and Proteomic Analysis
Abstract
:1. Introduction
2. Results
2.1. 1H-NMR Cellular Extract Signature of the Three Types of Cell Lines with Acquired Resistance
2.1.1. Identification of the Discriminant Metabolites Using Multivariate Data Analysis
2.1.2. Additional Spectral Investigations
2.1.3. Independent Evaluation of the NRAS, cKIT, and BRAF Resistance Signatures
2.2. 1H-NMR Extracellular Signature Investigations
2.3. Protein Microarray and Interactome Analyses
2.4. Integration of Metabonomics and Protein Microarray Data
3. Discussion
4. Materials and Methods
4.1. Cell Lines and Culture
4.2. Effectors
4.3. 1H-NMR Spectroscopy
4.4. Protein Microarray
4.5. Protein–Protein Interactions (PPI)
4.6. MSEA and JOINT Pathway Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Soumoy, L.; Schepkens, C.; Krayem, M.; Najem, A.; Tagliatti, V.; Ghanem, G.E.; Saussez, S.; Colet, J.-M.; Journe, F. Metabolic Reprogramming in Metastatic Melanoma with Acquired Resistance to Targeted Therapies: Integrative Metabolomic and Proteomic Analysis. Cancers 2020, 12, 1323. https://doi.org/10.3390/cancers12051323
Soumoy L, Schepkens C, Krayem M, Najem A, Tagliatti V, Ghanem GE, Saussez S, Colet J-M, Journe F. Metabolic Reprogramming in Metastatic Melanoma with Acquired Resistance to Targeted Therapies: Integrative Metabolomic and Proteomic Analysis. Cancers. 2020; 12(5):1323. https://doi.org/10.3390/cancers12051323
Chicago/Turabian StyleSoumoy, Laura, Corentin Schepkens, Mohammad Krayem, Ahmad Najem, Vanessa Tagliatti, Ghanem E. Ghanem, Sven Saussez, Jean-Marie Colet, and Fabrice Journe. 2020. "Metabolic Reprogramming in Metastatic Melanoma with Acquired Resistance to Targeted Therapies: Integrative Metabolomic and Proteomic Analysis" Cancers 12, no. 5: 1323. https://doi.org/10.3390/cancers12051323
APA StyleSoumoy, L., Schepkens, C., Krayem, M., Najem, A., Tagliatti, V., Ghanem, G. E., Saussez, S., Colet, J. -M., & Journe, F. (2020). Metabolic Reprogramming in Metastatic Melanoma with Acquired Resistance to Targeted Therapies: Integrative Metabolomic and Proteomic Analysis. Cancers, 12(5), 1323. https://doi.org/10.3390/cancers12051323