Biology of Oestrogen-Receptor Positive Primary Breast Cancer in Older Women with Utilisation of Core Needle Biopsy Samples and Correlation with Clinical Outcome
Abstract
:1. Introduction
- Consideration of standard clinicopathological features in the cohort.
- Measurement of a panel of biomarkers and correlation of findings with BCSS.
- Performing cluster analysis of the measured biomarkers and correlation of findings with BCSS.
2. Results
2.1. Clinicopathological Features
2.2. Panel of Biomarkers
2.3. Panel of Biomarkers—Correlation with BCSS
2.4. Cluster Analysis
2.5. Cluster Analysis—Correlation with BCSS
3. Discussion
3.1. Summary of the Findings
3.2. CNB TMA
3.3. Clinicopathological Features
3.4. Measurement of Panel of Biomarkers and Correlation with Clinical Outcome
3.5. Cluster Analysis and Correlation with Clinical Outcome
3.6. Strengths of the Study
3.7. Weaknesses of the Study
3.8. Future Directions
4. Materials and Methods
4.1. Patient Group
4.2. Construction of TMA
4.3. Clinicopathological Features
4.4. Measurement of Panel of Biomarkers
4.5. Cluster Analysis
4.6. Correlation with BCSS
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Characteristic | Number of Patients | Percentage |
---|---|---|
Age (years) | ||
70–79 | 294 | 55 |
≥80 | 242 | 45 |
Year of diagnosis | ||
1984–1989 | 4 | 1 |
1990–1994 | 5 | 1 |
1995–1999 | 174 | 32 |
2000–2004 | 252 | 47 |
2005–2010 | 101 | 19 |
Clinical size of tumour (cm) | ||
0.1–2 | 122 | 23 |
2.1–5 | 162 | 30 |
Unknown | 252 | 47 |
Grade (from core needle biopsy) | ||
1 | 62 | 12 |
2 | 206 | 39 |
3 | 30 | 6 |
Unknown | 238 | 44 |
Primary treatment | ||
Primary endocrine therapy | 333 | 62 |
Surgery | 189 | 35 |
Unknown | 14 | 3 |
Stage (Surgery group N = 189) | ||
1 | 93 | 49 |
2 | 63 | 33 |
3 | 14 | 7 |
Unknown | 19 | 10 |
Biomarker | Total Samples Tested (N) | Positive Expression (%) |
---|---|---|
PgR | 472 | 78 |
CK 7/8 | 499 | 99.6 |
BRCA1 | 400 | 70 |
VEGF | 468 | 88 |
Ki67 | 471 | 16 |
EGFR | 479 | 2 |
HER2 | 455 | 0.2 |
HER3 | 498 | 33 |
HER4 | 476 | 30 |
P53 | 477 | 11 |
CK5/6 | 507 | 12 |
MUC1 | 468 | 23 |
LKB1 | 446 | 31 |
BCL-2 | 477 | 45 |
PTEN | 474 | 15 |
AIB1 | 462 | 46 |
Biomarker | p-Value | Hazard Ratio | 95% CI |
---|---|---|---|
PgR | 0.015 | 0.432 | 0.219–0.850 |
Ki67 | 0.001 | 3.129 | 1.620–6.042 |
VEGF | 0.209 | 0.633 | 0.311–1.291 |
MUC1 | 0.033 | 2.051 | 1.058–3.976 |
P53 | 0.064 | 2.058 | 0.960–4.411 |
BCL-2 | 0.070 | 0.539 | 0.276–1.051 |
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Parks, R.M.; Albanghali, M.; Syed, B.M.; Green, A.R.; Ellis, I.O.; Cheung, K.-L. Biology of Oestrogen-Receptor Positive Primary Breast Cancer in Older Women with Utilisation of Core Needle Biopsy Samples and Correlation with Clinical Outcome. Cancers 2020, 12, 2067. https://doi.org/10.3390/cancers12082067
Parks RM, Albanghali M, Syed BM, Green AR, Ellis IO, Cheung K-L. Biology of Oestrogen-Receptor Positive Primary Breast Cancer in Older Women with Utilisation of Core Needle Biopsy Samples and Correlation with Clinical Outcome. Cancers. 2020; 12(8):2067. https://doi.org/10.3390/cancers12082067
Chicago/Turabian StyleParks, Ruth M., Mohammad Albanghali, Binafsha M. Syed, Andrew R. Green, Ian O. Ellis, and Kwok-Leung Cheung. 2020. "Biology of Oestrogen-Receptor Positive Primary Breast Cancer in Older Women with Utilisation of Core Needle Biopsy Samples and Correlation with Clinical Outcome" Cancers 12, no. 8: 2067. https://doi.org/10.3390/cancers12082067