Single-Cell RNA-seq Reveals Obesity-Induced Alterations in the Brca1-Mutated Mammary Gland Microenvironment
Abstract
:1. Introduction
2. Results
2.1. High-Fat Diets Induce Obesity in Brca1−/−; p53+/− Mice for scRNA-seq Analysis
2.2. The scRNA-Seq Analysis Identifies Various Molecular Subtypes of Mammary Stromal Fibroblasts in Brca1−/−; p53+/− mice
2.3. Obesity Aberrantly Modulates the Transcriptomic Landscape of Brca1−/−; p53+/− Mammary Stromal Fibroblasts to Alter their Functions in the Extracellular Matrix and Immunological Regulation
2.4. Obesity Aberrantly Regulates the Immune Ecosystem within Brca1−/−; p53+/− Mammary Glands to Establish a Tumor-Favorable Tissue Microenvironment
3. Discussion
4. Materials and Methods
4.1. HFD-Induced Obesity in Brca1−/−; p53+/− Mice
4.2. Single-Cell Isolation of Mammary Stromal and Immune Cells
4.3. Single-Cell RNA Sequencing and Data Analysis
4.4. Statistics and Reproducibility
5. Conclusions
DVA/US Government Disclaimer
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lo, P.-K.; Yao, Y.; Zhou, Q. Single-Cell RNA-seq Reveals Obesity-Induced Alterations in the Brca1-Mutated Mammary Gland Microenvironment. Cancers 2020, 12, 2235. https://doi.org/10.3390/cancers12082235
Lo P-K, Yao Y, Zhou Q. Single-Cell RNA-seq Reveals Obesity-Induced Alterations in the Brca1-Mutated Mammary Gland Microenvironment. Cancers. 2020; 12(8):2235. https://doi.org/10.3390/cancers12082235
Chicago/Turabian StyleLo, Pang-Kuo, Yuan Yao, and Qun Zhou. 2020. "Single-Cell RNA-seq Reveals Obesity-Induced Alterations in the Brca1-Mutated Mammary Gland Microenvironment" Cancers 12, no. 8: 2235. https://doi.org/10.3390/cancers12082235