Loss of Proprotein Convertase Furin in Mammary Gland Impairs proIGF1R and proIR Processing and Suppresses Tumorigenesis in Triple Negative Breast Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Results
2.1. Furin Is Highly Overexpressed in TNBC Tumors and Correlates with Poor Prognosis in TNBC Patients
2.2. Targeted Inactivation of Furin in the Mammary Gland via WAP-Cre Induces Normal Mammary Development
2.3. Inactivation of Furin in the Mammary Gland Inhibits Tumor Growth and Lung Metastasis in PyMT Induced TNBC Mice
2.4. Furin Inactivation in the Mammary Gland of TNBC Mice Inhibits the Proteolytic Maturation of proIGF1R and proIR and Downregulates PI3K/AKT and MAPK/ERK1/2 Expression and Activity
3. Discussion
4. Materials and Methods
4.1. Mouse Model
4.2. Animal Study
4.3. Bioinformatics Assay
4.4. Whole-Mount Staining of Mammary Glands
4.5. H&E Staining and Bouin’s Solution Staining
4.6. Quantitative RT-PCR
4.7. Isolation of Tumor Samples and Immunoblotting Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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He, Z.; Khatib, A.-M.; Creemers, J.W.M. Loss of Proprotein Convertase Furin in Mammary Gland Impairs proIGF1R and proIR Processing and Suppresses Tumorigenesis in Triple Negative Breast Cancer. Cancers 2020, 12, 2686. https://doi.org/10.3390/cancers12092686
He Z, Khatib A-M, Creemers JWM. Loss of Proprotein Convertase Furin in Mammary Gland Impairs proIGF1R and proIR Processing and Suppresses Tumorigenesis in Triple Negative Breast Cancer. Cancers. 2020; 12(9):2686. https://doi.org/10.3390/cancers12092686
Chicago/Turabian StyleHe, Zongsheng, Abdel-Majid Khatib, and John W. M. Creemers. 2020. "Loss of Proprotein Convertase Furin in Mammary Gland Impairs proIGF1R and proIR Processing and Suppresses Tumorigenesis in Triple Negative Breast Cancer" Cancers 12, no. 9: 2686. https://doi.org/10.3390/cancers12092686