Differential Expression of BOC, SPOCK2, and GJD3 Is Associated with Brain Metastasis of ER-Negative Breast Cancers
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Tissue Sample Selection
2.2. Morphological Assessment
2.3. Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
2.4. Immunohistochemistry
2.5. Statistics
3. Results
3.1. Identification of Genes Involved in Brain Metastasis of Primary Breast Cancer
3.2. Validations at mRNA and Protein Level
3.2.1. BOC mRNA Expression and Immunohistochemistry
3.2.2. SPOCK2 mRNA Expression and Immunohistochemistry
3.2.3. GJD3 mRNA Expression and Immunohistochemistry
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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# |
Age at Diagnosis (Years) | ER | PR | Her 2/neu | Lymph Node Status |
Neoadjuvant Therapy |
Adjuvant Therapy |
Metastasis Free-Period (Months) | Time to BM (Months) | 1st Metastatic Site | Other Metastatic Sites |
---|---|---|---|---|---|---|---|---|---|---|---|
1 | 32 | neg | neg | neg | pos | - | CT + RT | 6 | 11 | liver | lung, brain |
2 | 47 | neg | neg | neg | pos | - | CT + RT | 10 | - | skin | - |
3 | 53 | neg | neg | neg | pos | - | CT + RT | 48 | - | skin | - |
4 | 44 | neg | neg | neg | pos | - | CT + RT | 33 | - | lung | - |
5 | 42 | neg | neg | neg | pos | - | CT + RT | 24 | - | liver | - |
6 | 53 | neg | neg | neg | pos | - | CT + RT | 42 | 48 | skin | brain |
7 | 52 | neg | neg | neg | pos | - | CT + RT | 48 | 34 | lung | brain, lung |
8 | 59 | neg | neg | neg | pos | - | CT + RT | 35 | - | pleura | bone, liver, lung, meninges |
9 | 42 | neg | neg | neg | pos | - | CT + RT | 84 | - | bone | liver |
10 | 39 | neg | neg | neg | pos | - | CT + RT | 21 | 19 | skin | brain |
11 | 55 | neg | neg | neg | neg | - | CT + RT | 14 | - | liver | bone |
12 | 44 | neg | neg | neg | pos | - | CT + RT | 14 | 11 | brain | meninges, pleura |
13 | 61 | neg | neg | neg | pos | - | CT + RT | 26 | 56 | bone, lung | liver, brain |
14 | 64 | neg | neg | neg | pos | - | CT + RT | 7 | - | lung | liver, bone |
15 | 54 | neg | neg | neg | pos | - | CT + RT | 17 | - | liver | skin, bone, leptomengieal |
16 | 37 | neg | neg | neg | neg | - | CT + RT | 18 | - | lung, liver, bone | |
17 | 51 | neg | neg | neg | neg | - | CT + RT | 15 | - | bone | lung, skin, liver |
18 | 49 | neg | neg | neg | neg | - | CT + RT | 24 | - | bone | lung |
19 | 61 | neg | neg | neg | neg | - | CT + RT | 24 | 37 | lung | brain |
20 | 39 | neg | neg | pos | neg | - | CT | 51 | 95 | lung | brain, bone |
21 | 33 | neg | neg | neg | pos | - | CT + RT | 14 | - | lung, | liver, adrenal |
22 | 46 | neg | neg | neg | pos | - | CT + RT | 17 | - | bone | bone, lung, liver |
23 | 42 | neg | neg | neg | pos | - | CT | 51 | - | lung | liver |
24 | 63 | neg | neg | neg | pos | - | CT + RT | 42 | - | bone | - |
25 | 40 | neg | neg | neg | neg | - | CT + RT | 4 | 66 | lung | brain |
26 | 34 | neg | neg | neg | pos | - | CT + RT | 9 | - | liver | bone |
27 | 32 | neg | neg | neg | pos | N.A. | N.A. | 12 | - | skin | - |
28 | 25 | neg | neg | - | neg | - | CT + RT | 115 | 130 | bone | liver, brain |
29 | 53 | neg | neg | neg | pos | - | CT + RT | 15 | - | pleura | - |
30 | 33 | neg | neg | neg | pos | - | CT + RT | 34 | - | lung | liver, adrenal |
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Pedrosa, R.M.S.M.; Wismans, L.V.; Sinke, R.; van der Weiden, M.; van Eijck, C.H.J.; Kros, J.M.; Mustafa, D.A.M. Differential Expression of BOC, SPOCK2, and GJD3 Is Associated with Brain Metastasis of ER-Negative Breast Cancers. Cancers 2021, 13, 2982. https://doi.org/10.3390/cancers13122982
Pedrosa RMSM, Wismans LV, Sinke R, van der Weiden M, van Eijck CHJ, Kros JM, Mustafa DAM. Differential Expression of BOC, SPOCK2, and GJD3 Is Associated with Brain Metastasis of ER-Negative Breast Cancers. Cancers. 2021; 13(12):2982. https://doi.org/10.3390/cancers13122982
Chicago/Turabian StylePedrosa, Rute M. S. M., Leonoor V. Wismans, Renata Sinke, Marcel van der Weiden, Casper H. J. van Eijck, Johan M. Kros, and Dana A. M. Mustafa. 2021. "Differential Expression of BOC, SPOCK2, and GJD3 Is Associated with Brain Metastasis of ER-Negative Breast Cancers" Cancers 13, no. 12: 2982. https://doi.org/10.3390/cancers13122982