MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Establishment of Xenograft
2.2. Cell Cultures
2.3. Microscope Bioimaging
2.4. Western Blot
2.5. Flow Cytometry
2.6. Quantitative-Real-Time-PCR (QRT-PCR)
2.7. Statistical Analysis
3. Results
3.1. MM-129 Has Beneficial Effects in Eliminating Colon Cancer
3.2. MM-129 Inhibits the Intracellular Tumor-Promoting Pathway
3.3. MM-129 Evokes Cell Cycle Arrest via Upregulation of p53 and Downregulation of CDK2
3.4. MM-129 Decreases PD-L1 Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hermanowicz, J.M.; Pawlak, K.; Sieklucka, B.; Czarnomysy, R.; Kwiatkowska, I.; Kazberuk, A.; Surazynski, A.; Mojzych, M.; Pawlak, D. MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer. Cancers 2021, 13, 3203. https://doi.org/10.3390/cancers13133203
Hermanowicz JM, Pawlak K, Sieklucka B, Czarnomysy R, Kwiatkowska I, Kazberuk A, Surazynski A, Mojzych M, Pawlak D. MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer. Cancers. 2021; 13(13):3203. https://doi.org/10.3390/cancers13133203
Chicago/Turabian StyleHermanowicz, Justyna Magdalena, Krystyna Pawlak, Beata Sieklucka, Robert Czarnomysy, Iwona Kwiatkowska, Adam Kazberuk, Arkadiusz Surazynski, Mariusz Mojzych, and Dariusz Pawlak. 2021. "MM-129 as a Novel Inhibitor Targeting PI3K/AKT/mTOR and PD-L1 in Colorectal Cancer" Cancers 13, no. 13: 3203. https://doi.org/10.3390/cancers13133203